Evidence for Na+/Ca2+ exchanger 1 association with caveolin-1 and-2 in C6 glioma cells
Evidence for Na+/Ca2+ exchanger 1 association with caveolin-1 and-2 in C6 glioma cells
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DOI：
10.1080/15216540400020353
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发表时间：
2004-10-01
期刊：
影响因子：
4.6
通讯作者：
Suh, CK
中科院分区：
文献类型：
--
作者：
Cha, SH;Shin, SY;Suh, CK
本研究的目的是了解Cav-3缺失细胞（大鼠C6胶质瘤细胞）中Ca ~（2+）稳态的重要调节因子之一Na ~+-Ca ~（2+）交换蛋白（NCX 1）与Cav-1和Cav-2的相互作用。C6胶质瘤细胞中NCX 1、Cav-1和Cav-2的mRNA和蛋白均有表达，Cav-3的mRNA和蛋白无表达。在分离的小窝富集膜组分中，NCX 1、Cav-1和Cav-2蛋白定位于相同组分中。免疫沉淀实验显示NCX 1与Cavs之间形成复合物。共聚焦显微镜也支持NCX 1和Cavs在质膜上的共定位。在功能上，Cav-1反义ODN减弱了无钠诱导的NCX 1正向模式。Cav-2反义ODN处理细胞后，NCX 1的正向和反向表达均减弱。从这些结果来看，在Cav-3缺乏的细胞中，NCX 1的功能可能是通过与Cav-3结合来调节的。考虑到反义ODNs对NCX 1活性的抑制作用，caveolins可能在体内多种NCX 1相关疾病的病理生理过程中发挥重要作用。
The purpose of this study is to understand the interaction of Na+-Ca2+ exchanger (NCX1), that is one of the essential regulators of Ca2+ homeostasis, with caveolin (Cav)-1 and Cav-2 in Cav-3 null cell (rat C6 glioma cell). Both mRNA and protein expression of NCX1, Cav-1 and Cav-2 was observed, but no expression of mRNA and protein of Cav-3 were observed in C6 glioma cells. In isolated caveolae-enriched membrane fraction, the NCX1, Cav-1 and Cav-2 proteins localized in same fractions. The experiment of immuno-precipitation showed complex formation between the NCX1 and Cavs. Confocal microscopy also supported co-localization of NCX1 and Cavs at the plasma membrane. Functionally, sodium-free induced forward mode of NCX1 attenuated by Cav-1 antisense ODN. When treated cells with Cav-2 antisense ODN, both reverse and forward mode of NCX1 was attenuated. From these results, in the Cav-3 lacking cells, the function of NCX1 might be regulated by binding with Cavs. Considering the decrement of NCX1 activity by antisense ODNs, caveolins may play an important role in diverse of pathophysiological process of NCX1-related disorders in the body.