Cationic liposomes enhanced firefly bioluminescent assay of adenosine 5'-triphosphate disodium salt.
Cationic liposomes enhanced firefly bioluminescent assay of adenosine 5'-triphosphate disodium salt.
复制标题
阳离子脂质体增强腺苷 5-三磷酸二钠盐的萤火虫生物发光测定。
DOI：
10.1006/abio.1996.9832
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发表时间：
1997
影响因子：
2.9
通讯作者：
H. Watanabe
中科院分区：
文献类型：
--
作者：
T. Kamidate;T. Kinkou;H. Watanabe
含有硬脂胺的阳离子脂质体在1.0 PM至5.5 nm的范围内增强了萤火虫荧光素-荧光素酶与ATP反应的最大发光强度。相反，在阴离子和两性离子脂质体存在下的生物发光强度与单独在水中的生物发光强度大致相同。在阳离子脂质体存在下，三磷酸腺苷在标准水溶液中的检出限为1.0 pM。与单独在水中相比，对ATP的灵敏度提高了5倍。将该方法用于大肠杆菌提取物中三磷酸腺苷的测定。推测其灵敏度阈值为340个细胞ml-1。
Cationic liposomes containing stearylamine enhanced the intensity of maximum light emission from the firefly luciferin-luciferase reaction with ATP in the range of 1.0 pM up to 5.5 nM. In contrast, the bioluminescent intensities in the presence of anionic and zwitterionic liposomes were about the same as those in water alone. The detection limit for ATP in the presence of cationic liposomes was 1.0 pM in aqueous ATP standard solutions. The sensitivity for ATP was improved by a factor of five times compared to that in water alone. The method was applied to the determination of ATP in Escherichia coli extracts. The sensitivity threshold was presumed to be 340 cells ml-1.