Nationwide molecular surveillance of exfoliative toxigenic Staphylococcus hyicus on pig farms across Japan
Nationwide molecular surveillance of exfoliative toxigenic Staphylococcus hyicus on pig farms across Japan
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DOI：
10.1016/j.vetmic.2007.04.036
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发表时间：
2007-10-06
影响因子：
3.3
通讯作者：
Fukuyasu, Tsuguaki
中科院分区：
文献类型：
--
作者：
Futagawa-Saito, Keiko;Ba-Thein, William;Fukuyasu, Tsuguaki
我们调查了携带葡萄球菌hyicus和他们的剥脱毒素基因（exhA，exhB，exhC，exhD，和shetb在424头猪从38个日本猪场的PCR和特征的分离物的16 S-23 S间隔区多态性分析。S.从17.9%的猪中分离到猪嗜血杆菌207株，其中72.5%为致病性。致突变性S.渗出性表皮炎猪的Hyicus是健康猪的4倍（87.6%比19.6%; P < 0.01）。在毒素中，exhA是最普遍的，在49.3%的毒素基因阳性菌株中可检测到。S. 207株猪蜱分离株可分为9个ITS-PCR型（a-i），其中以d、h、e型为主，占总分离株的70%（145/207），占毒力基因阳性菌株的76%（114/150）。该研究表明，在S.猪流感病毒在日本的养猪场流行。(c)2007年由Elsevier B. V.出版。
We investigated the carriage of Staphylococcus hyicus and their exfoliative toxin genes (exhA, exhB, exhC, exhD, and shetb) among 424 pigs from 38 Japanese pig farms by PCR and characterized the isolates by 16S-23S intergenic spacer region polymorphism analysis. S. hyicus (n = 207) were isolated from 17.9% of pigs and 72.5% of them were toxigenic. The isolation rate of toxigenic S. hyicus was four times higher in the pigs with exudative epidermitis than the healthy pigs (87.6% versus 19.6%; p < 0.01). Among the toxins, exhA was the most prevalent, being detectable in 49.3% of toxin gene-positive strains. S. hyicus isolates were distributed into nine ITS-PCR types (a-i), with ITS-PCR types d, h, and e being predominant accounting for 70% (145/207) of total isolates or 76% (114/150) of toxin gene-positive strains. This study denotes high prevalence of toxigenic strains among S. hyicus circulating on pig farms in Japan. (c) 2007 Published by Elsevier B.V.