Pharmacokinetic profile of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in mice after oral administration of Polygonum multiflorum extract
Pharmacokinetic profile of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in mice after oral administration of Polygonum multiflorum extract
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DOI：
10.3109/03639045.2011.597763
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Shan, Letian
中科院分区：
文献类型：
--
作者：
Lv, Guiyuan;Gu, Hui;Shan, Letian
内容：二苯乙烯苷（2，3，5，4 '-tetrahydroxystilbene-2-O-beta-D-glucoside）是何首乌的主要活性成分，是临床上常用的抗衰老中药。目的：研究何首乌二苯乙烯苷（PM-SG）在小鼠体内的药代动力学，定量测定小鼠口服100 mg/kg何首乌提取物后血浆中PM-SG的浓度。材料与方法：采用反相高效液相色谱-液-液相萃取联用技术，研究了该方法的灵敏度。应用房室和非房室分析在小鼠中测定PM-SG的药代动力学参数。血浆中PM-SG的标准曲线在0.66 - 56.40 μ g/ml范围内呈线性关系（r（2）> 0.99），并绘制浓度-时间曲线，最大浓度（C-max）和达到最大浓度的时间（T-max）为29.62 μ g/ml。ml和60 min。方法的相对标准偏差（RSD）和相对误差（RE）均小于3%，回收率大于97
Context: Stilbene glycoside (2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside) is a main bioactive component of Polygonum multiflorum, a traditional Chinese medicine (TCM) commonly used in clinic for anti-aging treatment. Its medicinal activities, such as anti-oxidation, anti-inflammation and endothelial protection, have been extensively studied, but its pharmacokinetic property is still unclear.Objective: A pharmacokinetic study was undertaken to quantitatively determine P. multiflorum stilbene glycoside (PM-SG) in mouse plasma after oral administration of 100 mg/kg P. multiflorum extract. Materials and methods: A sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with liquid-liquid phase extraction method was employed for this study. Pharmacokinetic parameters of PM-SG were determined in mice applying both compartmental and non-compartmental analyses.Results and discussion: The calibration curve for PM-SG in the plasma was linear (r(2) > 0.99) over the range of 0.66 to 56.40 mu g/ml, and the concentration-time curve was plotted with the maximum concentration (C-max) and time to reach maximum concentration (T-max) of 29.62 mu g/ml and 60 min, respectively. The intra-and inter-day variations were less than 3% for relative standard deviation (RSD) and relative error (RE), with a good recovery of more than 97% (RSD