Co-localization of p-CREB and p-NR1 in spinothalamic neurons in a chronic muscle pain model
Co-localization of p-CREB and p-NR1 in spinothalamic neurons in a chronic muscle pain model
复制标题
DOI：
10.1016/j.neulet.2007.02.078
复制
发表时间：
2007-05-11
影响因子：
2.5
通讯作者：
Sluka, Kathleen A.
中科院分区：
文献类型：
--
作者：
Bement, Marie K. Hoeger;Sluka, Kathleen A.
cAMP通路的激活是慢性肌肉疼痛的重要介质。本研究检测了脊髓中转录因子cAMP反应元件结合蛋白（p-CREB）和NMDA受体NR 1亚基（p-NR 1）的磷酸化。通过向雄性Sprague-Dawley大鼠的腓肠肌中注射两次酸性盐水（间隔5天）来诱导爪的双侧机械性痛觉过敏。与接受pH 7.2注射的动物相比，第二次肌肉注射酸后24小时，脊髓丘脑神经元表达p-NR 1或p-CREB的比例在背角中没有变化。背角中脊髓丘脑神经元的这种缺乏变化可能是由于单个脊髓丘脑神经元的增加或非脊髓丘脑神经元的增加。在X板层中，表达p-NR 1的脊髓丘脑神经元的比例增加。这些结果表明，有特定区域的变化，脊髓丘脑神经元表达p-NR 1和X板层可能在慢性肌肉疼痛的调制中发挥重要作用。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Activation of the cAMP pathway is an important mediator of chronic muscle pain. This study examined phosphorylation of the transcription factor cAMP-response-element-binding protein (p-CREB) and the NR1 subunit of the NMDA receptor (p-NR1) in the spinal cord. Bilateral mechanical hyperalgesia of the paw was induced by administering two injections of acidic saline, 5 days apart, into the gastrocnemius muscle of male Sprague-Dawley rats. The proportion of spinothalamic neurons that expressed p-NR1 or p-CREB did not change in the dorsal horn 24 h after the second intramuscular acid injection compared with animals that received pH 7.2 injections. This lack of change in spinothalamic neurons in the dorsal horn may be due to increases in individual spinothalamic neurons or increases in non-spinothalamic neurons. There was an increase in the proportion of spinothalamic neurons expressing p-NR1 in lamina X. These findings suggest that there are region-specific changes in spinothalamic neurons that express p-NR1 and lamina X may play an important role in the modulation of chronic muscle pain. (c) 2007 Elsevier Ireland Ltd. All rights reserved.