EFFECT OF GLUTAMINE SUPPLEMENT AND HEPATECTOMY ON DNA AND PROTEIN-SYNTHESIS IN THE REMNANT LIVER
EFFECT OF GLUTAMINE SUPPLEMENT AND HEPATECTOMY ON DNA AND PROTEIN-SYNTHESIS IN THE REMNANT LIVER
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DOI：
10.1006/jsre.1995.1194
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发表时间：
1995-10-01
影响因子：
2.2
通讯作者：
KAKEGAWA, T
中科院分区：
文献类型：
--
作者：
YOSHIDA, S;YUNOKI, T;KAKEGAWA, T
谷氨酰胺的生理作用之一是作为 DNA 合成的前体。本研究的目的是确定 (1) 补充谷氨酰胺的全肠外营养 (TPN) 是否增强肝脏中 DNA 和蛋白质的合成，以及 (2) 大鼠部分肝切除术后谷氨酰胺的摄取是否增加。雄性Donryu大鼠（n = 59；体重250-275 g）随机分为四组：（1）假手术+标准TPN溶液（C-STPN）；（2）假手术+标准TPN溶液（C-STPN）； (2) C+谷氨酰胺补充的TPN(C-GTPN)； （3）70%肝部分切除+STPN（H-STPN）； (4)肝部分切除+GTPN(H-GTPN)。第0天，大鼠接受假手术或70%部分肝切除术，同时在颈静脉中插入导管。手术后立即开始 TPN。 GTPN 与 STPN 是等热量和等氮的，总氮的 25% 以谷氨酰胺形式提供。第2天，在连续输注1-C-14-亮氨酸或静脉内推注溴脱氧尿苷后处死动物。补充谷氨酰胺可提高肝脏再生率（H-STPN，60.8 +/- 1.6% vs H-GTPN，66.3 +/- 2.0，P < 0.05），因为肝脏中蛋白质合成增加（H-STPN，134.0 +/- 10.3%/天 vs H-GTPN，160.9 +/- 6.9，P < 0.05） 0.05）和肝细胞中的 DNA 合成（H-STPN，23.1 +/- 2.5% vs H-GTPN，31.4 +/- 2.9，P < 0.05）。肝切除术后补充 GTPN 后，肝脏对谷氨酰胺的吸收增加。总之，谷氨酰胺补充和部分肝切除刺激了肝脏中谷氨酰胺的摄取，这导致残余肝脏中蛋白质和 DNA 合成的增强。 (C) 1995 学术出版社
One of the physiological roles of glutamine is as a precursor for DNA synthesis. The aims of this study were to determine (1) whether glutamine-supplemented total parenteral nutrition (TPN) enhanced DNA and protein synthesis in the liver and (2) whether glutamine uptake was increased following partial hepatectomy in rats. Male Donryu rats (n = 59; body weight, 250-275 g) were randomized into four groups: (1) sham operation + standard TPN solution (C-STPN); (2) C + glutamine-supplemented TPN (C-GTPN); (3) 70% partial hepatectomy + STPN (H-STPN); (4) partial hepatectomy + GTPN (H-GTPN). On Day 0, rats underwent either a sham operation or 70% partial hepatectomy and concomitantly were catheterized in the jugular vein. TPN was begun immediately after the surgery. GTPN was isocaloric and isonitrogenous with STPN and 25% of total nitrogen was given as glutamine. On Day 2, the animals were sacrificed after either a continuous infusion of 1-C-14-leucine or a bolus iv injection of bromodeoxyuridine. The rate of hepatic regeneration was enhanced with glutamine supplementation (H-STPN, 60.8 +/- 1.6% vs H-GTPN, 66.3 +/- 2.0, P < 0.05) due to an increase in protein synthesis in the liver (H-STPN, 134.0 +/- 10.3%/day vs H-GTPN, 160.9 +/- 6.9, P < 0.05) and DNA synthesis in hepatocytes (H-STPN, 23.1 +/- 2.5% vs H-GTPN, 31.4 +/- 2.9, P < 0.05). The uptake of glutamine by the liver was increased following hepatectomy with GTPN supplementation. In conclusion, glutamine supplement and partial hepatectomy stimulated glutamine uptake in the liver and this results in an enhancement of protein and DNA synthesis in the remnant liver. (C) 1995 Academic Press, Inc.