Elevated LINC01550 induces the apoptosis and cell cycle arrest of melanoma
Elevated LINC01550 induces the apoptosis and cell cycle arrest of melanoma
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LINC01550 升高诱导黑色素瘤细胞凋亡和细胞周期停滞
DOI：
10.1007/s12032-021-01478-x
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发表时间：
2021-04-01
期刊：
影响因子：
3.4
通讯作者：
Zhou, Jianda
中科院分区：
文献类型：
--
作者：
Chen, Jia;Li, Ping;Zhou, Jianda
黑色素瘤是人类皮肤癌中死亡率最高的高度恶性亚型。在这里，我们利用基因表达总览(GEO)数据库和基因表达谱交互分析(GEPIA)平台对人黑色素瘤组织进行生物信息学分析。我们发现，与正常组织相比，LINC01550在黑色素瘤组织中的表达显著下调。LINC01550的低表达与黑色素瘤患者总生存期和无病生存期的缩短密切相关。单细胞RNA测序数据库(scRNA-seq)证明，LINC01550的表达与黑色素瘤的肿瘤细胞增殖和侵袭能力呈负相关。将LINC01550高表达载体转入黑色素瘤细胞(WM35和WM451)。LINC01550表达上调可显著抑制黑色素瘤细胞的增殖和侵袭能力，诱导细胞凋亡，并使细胞发生G1期和S期阻滞。结论：LINC01550的过表达可能成为治疗黑色素瘤的潜在靶点。
Melanoma is a high-grade malignant subtype of human skin cancer with the highest mortality rate. Here we perform a bioinformatics analysis concerning human melanoma tissues by the Gene Expression Omnibus (GEO) database and Gene Expression Profiling Interactive Analysis (GEPIA) platform. We found that lncRNA LINC01550 was significantly down-regulated in the melanoma tissues as compared to the normal tissues. The low expression of LINC01550 was tightly associated with shorter overall survival and disease-free survival of patients with melanoma. LINC01550 expression is negatively associated with tumor cell proliferation and invasion abilities in melanoma as evidenced by the single-cell RNA sequencing (scRNA-seq) databases. LINC01550-overexpressing vectors were transferred into melanoma cells (WM35 and WM451). Up-regulation of LINC01550 significantly inhibited proliferation and invasion abilities, as well as induced cell apoptosis and G1 and S phase arrest of the melanoma cells. In conclusion, overexpression of LINC01550 may serve as a potential therapeutic target for melanoma.