Does leptin play a cytokine-like role within the airways of COPD patients?
Does leptin play a cytokine-like role within the airways of COPD patients?
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DOI：
10.1183/09031936.05.00092404
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发表时间：
2005-09-01
影响因子：
24.3
通讯作者：
Vignola, AM
中科院分区：
文献类型：
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作者：
Bruno, A;Chanez, P;Vignola, AM
慢性阻塞性肺疾病（COPD）患者气道瘦素-瘦素受体系统表达上调。在正常人和吸烟者的支气管活检中，有和没有COPD，本研究探讨瘦素和瘦素受体的表达和他们的共同定位在气道和炎症cells.Combining免疫组化与末端脱氧核苷酸转移酶dUTP缺口末端标记技术，在气道和炎症细胞和瘦素和瘦素受体表达细胞的凋亡进行了研究。在上皮细胞瘦素和瘦素受体的表达高于正常人比吸烟者和COPD患者。与此相反，在粘膜下层，瘦素过度表达COPD与正常人和吸烟者相比。瘦素及其受体主要与活化的T细胞（CD 45 RO）和CD 8（+）T淋巴细胞共定位，吸烟组部分炎性细胞出现凋亡，而COPD组炎性细胞中瘦素和瘦素受体阳性细胞均未出现凋亡。Leptin表达与COPD严重程度相关，并采用慢性阻塞性肺疾病全球倡议（Global initiative for Chronic Obstructive Lung Disease）分级进行评估。总之，本研究显示慢性阻塞性肺疾病患者支气管粘膜中Leptin表达增加，与气道炎症和气流阻塞相关。
The leptin-leptin receptor system might be up-regulated in the airways of chronic obstructive pulmonary disease (COPD). In bronchial biopsies obtained from normal subjects and smokers, with and without COPD, the present study examined leptin and leptin-receptor expression and their co-localisation in airway and inflammatory cells.Combining immunohistochemistry with terminal deoxynucleotidyl transferase dUTP nick end-labelling techniques, apoptosis in airway and inflammatory cells and in leptin and leptin-receptor expressing cells was investigated. In the epithelial cells both leptin and leptin-receptor expression was higher in normal subjects than in smokers and COPD subjects. By contrast, in the submucosa, leptin was over-expressed in COPD when compared with normal subjects and smokers. Leptin and its receptor were co-localised, mainly with activated T cells (CD45RO) and CD8(+) T lymphocytes.In smokers, apoptosis was found in some inflammatory cells, whereas in COPD inflammatory cells, leptin and leptin-receptor positive cells were not apoptotic. Leptin expression was related to COPD severity and assessed using the Global initiative for Chronic Obstructive Lung Disease classification.In conclusion, the present study shows an increased leptin expression in bronchial mucosa of chronic obstructive pulmonary disease patients, associated with airway inflammation and airflow obstruction.