Inhibition of basic fibroblast growth factor expression, angiogenesis, and growth of human bladder carcinoma in mice by systemic interferon-alpha administration.
Inhibition of basic fibroblast growth factor expression, angiogenesis, and growth of human bladder carcinoma in mice by systemic interferon-alpha administration.
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DOI：
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发表时间：
1998-02
期刊：
影响因子：
11.2
通讯作者：
C. Dinney;D. Bielenberg;P. Perrotte;R. Reich;B. Eve;C. Bucana;I. Fidler
中科院分区：
文献类型：
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作者：
C. Dinney;D. Bielenberg;P. Perrotte;R. Reich;B. Eve;C. Bucana;I. Fidler
这些研究的目的是确定全身给予IFN-α是否可以抑制人移行细胞癌中碱性成纤维细胞生长因子（bFGF）的表达，减少其血管生成，从而抑制其在裸鼠膀胱壁中的生长。高度转移的 253J B-V 细胞和 IFN-α 抗性 253J B-V IFNR 细胞与非细胞抑制浓度的 IFN-α 的体外培养下调了稳态 mRNA 转录物和 bFGF 的蛋白质产生。将IFN-α不敏感和IFN-α抗性细胞植入裸鼠膀胱壁。全身给予IFN-α可降低bFGF的体内表达，降低肿瘤中的血管密度，并抑制IFN-α不敏感和IFN-α抗性细胞的肿瘤生长。这些数据表明，除了其有据可查的抗增殖作用之外，IFN-α还可以通过抑制血管生成来抑制人膀胱癌细胞的生长。
The purpose of these studies was to determine whether systemic administration of IFN-alpha can inhibit the expression of basic fibroblast growth factor (bFGF) in human transitional cell carcinoma, reduce its angiogenesis, and thus inhibit its growth in the bladder wall of nude mice. In vitro incubation of the highly metastatic 253J B-V cells and the IFN-alpha-resistant 253J B-V IFNR cells with noncytostatic concentrations of IFN-alpha down-regulated the steady-state mRNA transcripts and protein production of bFGF. IFN-alpha-insensitive and IFN-alpha-resistant cells were implanted in the bladder wall of nude mice. Systemic administration of IFN-alpha decreased the in vivo expression of bFGF, decreased blood vessel density in the tumors, and inhibited tumor growth of both IFN-alpha-insensitive and IFN-alpha-resistant cells. These data suggest that in addition to its well-documented antiproliferative effects, IFN-alpha can inhibit the growth of human bladder cancer cells by inhibition of angiogenesis.