Spatial shifts in microbial population structure within poultry litter associated with physicochemical properties
Spatial shifts in microbial population structure within poultry litter associated with physicochemical properties
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DOI：
10.1093/ps/86.9.1840
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发表时间：
2007-09-01
期刊：
影响因子：
4.4
通讯作者：
Sistani, K.
中科院分区：
文献类型：
--
作者：
Lovanh, N.;Cook, K. L.;Sistani, K.
除了潜在的人类或牲畜病原体外，家禽粪便中的微生物种群在很大程度上被忽视了。更好地了解家禽窝中微生物种群的群落结构和特性有助于制定管理措施，从而减少造成有毒空气排放和病原体发病率的种群。本研究采用主成分分析（PCA）和变性梯度凝胶电泳（DGGE）方法分析了家禽凋落物空气和物理性质与微生物群落结构变化的相关性。在一个146米× 12.8米的鸡舍中，在5米宽、12米深的地方以36点的网格模式采集窝屑样本。在每个采样点，记录凋落物水分、pH、空气和凋落物温度、相对湿度等物理参数，并采集样本进行分子分析。DGGE分析表明，家禽舍背部和水/饲养区样品的条带分布与其他地区样品的条带分布明显不同。在前区、中前区、中后区、后区和水/饲料区有明显的带状格局。主成分分析显示出相似的聚类模式，但前房和中房样本的分离更为明显。主成分分析还表明，水分含量和凋落物温度（分别占样品分离量的51.5%和31.5%）对鸡舍微生物群落的空间多样性起主要作用。根据DGGE指纹图谱和克隆的DGGE条带序列分析，不同房屋长度的微生物类型存在差异，这与凋落物的物理性质存在差异。
Microbial populations within poultry litter have been largely ignored with the exception of potential human or livestock pathogens. A better understanding of the community structure and identity of the microbial populations within poultry litter could aid in the development of management practices that would reduce populations responsible for toxic air emissions and pathogen incidence. In this study, poultry litter air and physical properties were correlated to shifts in microbial community structure as analyzed by principal component analysis (PCA) and measured by denaturing gradient gel electrophoresis (DGGE). Litter samples were taken in a 36-points grid pattern at 5 m across and 12 m down a 146 m x 12.8 m chicken house. At each sample point, physical parameters such as litter moisture, pH, air and litter temperature, and relative humidity were recorded, and samples were taken for molecular analysis. The DGGE analysis showed that the banding pattern of samples from the back and water/feeder areas of poultry house were distinct from those of samples from other areas. There were distinct clusters of banding patterns corresponding to the front, middle front, middle back, back, and waterer /feeder areas. The PCA analysis showed similar cluster patterns, but with more distinct separation of the front and midhouse samples. The PCA analysis also showed that moisture content arid litter temperature (accounting for 51.5 and 31.5% of the separation of samples, respectively) play a major role in spatial diversity of microbial community in the poultry house. Based on analysis of DGGE fingerprints and cloned DGGE band sequences, there appear to be differences in the types of microorganisms over the length of the house, which correspond to differences in the physical properties of the litter.