The Crithidia fasciculata KAP1 gene encodes a highly basic protein associated with kinetoplast DNA
The Crithidia fasciculata KAP1 gene encodes a highly basic protein associated with kinetoplast DNA
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DOI：
10.1016/s0166-6851(98)00048-6
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发表时间：
1998-07-01
影响因子：
1.5
通讯作者：
Ray, DS
中科院分区：
文献类型：
--
作者：
Hines, JC;Ray, DS
束状短翅虫KAP 1基因编码一种与动基体DNA相关的小碱性蛋白（p21）。p21蛋白具有与靶向动基体的几种其它蛋白质的序列密切相关的9个氨基酸的可切割前序列，并且非特异性地结合动基体小环DNA。p21蛋白的计算pI也为13，其中两个氨基酸（赖氨酸和丙氨酸）占残基的50%以上，28个赖氨酸残基中有25个包含在蛋白质的C-末端一半中。p21的免疫定位显示，该蛋白质仅存在于动质体中，其定位与动质体DNA拓扑异构酶和DNA聚合酶的对足定位明显不同。KAP 1基因是单拷贝基因，并且KAPI mRNA在整个细胞周期中以恒定水平存在。这种高度碱性的蛋白质可能在动基体DNA的凝聚或分离中发挥作用，（C）1998 Elsevier Science B.V.保留所有权利。
The Crithidia fasciculata KAP1 gene encodes a small basic protein (p21) associated with kinetoplast DNA. The p21 protein has a nine amino acid cleavable presequence closely related to those of several other proteins targeted to the kinetoplast and binds non-specifically to kinetoplast minicircle DNA. The p21 protein also has a calculated pI of 13 with two amino acids (lysine and alanine) accounting for more than 50% of the residues and with 25 out of 28 lysine residues contained in the C-terminal half of the protein. Immunolocalization of p21 shows that the protein is found exclusively in the kinetoplast with a localization distinctly different from the antipodal localization of kinetoplast DNA topoisomerase and DNA polymerase, The KAP1 gene is a single copy gene and the KAPI mRNA is present at a constant level throughout the cell cycle. This highly basic protein may play a role in the condensation or segregation of the kinetoplast DNA, (C) 1998 Elsevier Science B.V. All rights reserved.