Coaggregation of Porphyromonas gingivalis and Prevotella intermedia
Coaggregation of Porphyromonas gingivalis and Prevotella intermedia
复制标题
DOI：
10.1111/j.1348-0421.2001.tb01298.x
复制
发表时间：
2001-01-01
影响因子：
2.6
通讯作者：
Baba, H
中科院分区：
文献类型：
--
作者：
Kamaguchi, A;Nakayama, K;Baba, H
牙龈卟啉单胞菌细胞与中间普雷沃菌细胞共聚集。用L-精氨酸、L-赖氨酸、N α-对甲苯磺酰基-L-赖氨酸氯甲基酮、胰蛋白酶抑制剂和亮抑酶肽抑制共聚集。热和蛋白酶K处理的牙龈卟啉单胞菌细胞没有显示与中间卟啉单胞菌细胞的共聚集，而热和蛋白酶K处理的中间卟啉单胞菌细胞没有影响共聚集。牙龈卟啉单胞菌培养上清中的囊泡与中间型卟啉单胞菌细胞发生聚集，加入L-精氨酸或L-赖氨酸以及热处理囊泡也能抑制这种聚集。牙龈卟啉单胞菌的rgpA rgpB、rgpA kgp、rgpA rgpB kgp和rgpA kgp hagA突变体不与中间卟啉单胞菌共聚集。另一方面，缺乏FimA菌毛的fimA突变体显示与P intermedia以及野生型亲本共聚集。这些结果强烈暗示牙龈卟啉单胞菌细胞表面上的热不稳定性和蛋白质因子，最有可能是牙龈卟啉蛋白酶-粘附素复合物，参与牙龈卟啉单胞菌和中间型卟啉单胞菌的共聚集。
Porphyromonas gingivalis cells coaggregated with Prevotella intermedia cells. The coaggregation was inhibited with L-arginine, L-lysine, N alpha -p-tosyl-L-lysine chloromethyl ketone, trypsin inhibitor, and leupeptin. Heat- and proteinase K-treated P. gingivalis cells showed no coaggregation with P. intermedia cells, whereas heat and proteinase K treatments of P intermedia cells did not affect the coaggregation. The vesicles from P. gingivalis culture supernatant aggregated with P intermedia cells, and this aggregation was also inhibited by addition of L-arginine or L-lysine and by heat treatment of the vesicles. The rgpA rgpB, rgpA kgp, rgpA rgpB kgp, and rgpA kgp hagA mutants of P. gingivalis did not coaggregate with P. intermedia. On the other hand, the fimA mutant lacking the FimA fimbriae showed coaggregation with P intermedia as well as the wild type parent. These results strongly imply that a heat-labile and proteinous factor on the cell surface of P. gingivalis, most likely the gingipain-adhesin complex, is involved in coaggregation of P. gingivalis and P. intermedia.