Biased hypermutation occurred frequently in a gene inserted into the IC323 recombinant measles virus during its persistence in the brains of nude mice
Biased hypermutation occurred frequently in a gene inserted into the IC323 recombinant measles virus during its persistence in the brains of nude mice
复制标题
IC323重组麻疹病毒在裸鼠大脑中持续存在期间，插入IC323重组麻疹病毒的基因经常发生偏向超突变
DOI：
10.1016/j.virol.2014.05.035
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ogura H
中科院分区：
文献类型：
--
作者：
Otani S;Ayata M;Takeuchi K;Takeda M;Shintaku H;Ogura H
麻疹病毒（MV）是麻疹及其神经系统并发症、亚急性硬化性全脑炎（SSPE）和麻疹包涵体脑炎（MIBE）的病原体。M基因的偏向性超突变是SSPE和MIBE的典型特征。为了确定M基因是否是超突变的优选靶标，将含有人源化肾海肾绿色荧光蛋白（hrGFP）基因的额外转录单位引入IC323 MV基因组中，并将病毒脑内接种裸鼠。当hrGFP基因插入前导基因和N基因之间时，在M基因和hrGFP基因中发生了偏性超突变，但在H和L基因之间没有发生偏性超突变。这些结果表明，偏向超突变通常发现在一个基因，其功能是不是必不可少的病毒在大脑中的增殖和MV基因组中的基因的位置可以影响其突变频率。
Measles virus (MV) is the causative agent of measles and its neurological complications, subacute sclerosing panencephalitis (SSPE) and measles inclusion body encephalitis (MIBE). Biased hypermutation in the M gene is a characteristic feature of SSPE and MIBE. To determine whether the M gene is the preferred target of hypermutation, an additional transcriptional unit containing a humanizedRenilla reniformisgreen fluorescent protein (hrGFP) gene was introduced into the IC323 MV genome, and nude mice were inoculated intracerebrally with the virus. Biased hypermutation occurred in the M gene and also in the hrGFP gene when it was inserted between the leader and the N gene, but not between the H and L gene. These results indicate that biased hypermutation is usually found in a gene whose function is not essential for viral proliferation in the brain and that the location of a gene in the MV genome can affect its mutational frequency.