Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis.
Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis.
复制标题
猪链球菌染色体 yefM-yoeB 毒素-抗毒素系统的鉴定和表征
DOI：
10.1038/srep13125
复制
发表时间：
2015-08-14
影响因子：
4.6
通讯作者：
Bei W
中科院分区：
文献类型：
--
作者：
Zheng C;Xu J;Ren S;Li J;Xia M;Chen H;Bei W
毒素-抗毒素（TA）系统广泛存在于细菌和古细菌的基因组中。这些模块已经在大肠杆菌和各种其他细菌中被鉴定。然而，它们存在于猪链球菌，一种重要的人畜共患病原体的基因组中，很少受到关注。在这项研究中，我们描述了一个II型TA系统，包括染色体yefM-yoeB基因座的S。猪。yefM-yoeB基因座存在于大多数S血清型的基因组中。猪。S. suis YoeB毒素抑制E. coli的毒力和S.抗猪链球菌YefM毒素可减轻YoeB的感染。猪链球菌和肺炎链球菌，而E. coli YefM.更重要的是，S。suis yefM-yoeB系统转化为E. coli对细胞生长有影响。在小鼠感染模型中，yefM-yoeB基因座的缺失对S.猪血清2型综合以上结果，我们的数据表明，S. suis是一个主动的TA系统，不涉及毒力。
Toxin-antitoxin (TA) systems are widely prevalent in the genomes of bacteria and archaea. These modules have been identified in Escherichia coli and various other bacteria. However, their presence in the genome of Streptococcus suis, an important zoonotic pathogen, has received little attention. In this study, we describe the identification and characterization of a type II TA system, comprising the chromosomal yefM-yoeB locus of S. suis. The yefM-yoeB locus is present in the genome of most serotypes of S. suis. Overproduction of S. suis YoeB toxin inhibited the growth of E. coli and the toxicity of S. suis YoeB could be alleviated by the antitoxin YefM from S. suis and Streptococcus pneumoniae, but not by E. coli YefM. More importantly, introduction of the S. suis yefM-yoeB system into E. coli could affect cell growth. In a murine infection model, deletion of the yefM-yoeB locus had no effect on the virulence of S. suis serotype 2. Collectively, our data suggested that the yefM-yoeB locus of S. suis is an active TA system without the involvement of virulence.