Microcircuit Rules Governing Impact of Single Interneurons on Purkinje Cell Output In Vivo
Microcircuit Rules Governing Impact of Single Interneurons on Purkinje Cell Output In Vivo
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DOI：
10.1016/j.celrep.2020.02.009
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发表时间：
2020-03-03
期刊：
影响因子：
8.8
通讯作者：
Hausser, Michael
中科院分区：
文献类型：
--
作者：
Arlt, Charlotte;Hausser, Michael
单个中间神经元对体内神经元输出的功能影响以及中间神经元如何通过生理活动模式被招募仍然知之甚少。在小脑皮质，分子层中间神经元和它们的目标，浦肯野细胞，从颗粒细胞和攀爬纤维接收兴奋性输入。使用双膜片钳记录从中间神经元和浦肯野细胞在体内，我们探测这些电路元件之间的时空相互作用。我们发现，单个中间神经元尖峰可以有效地抑制浦肯野细胞的输出，这取决于中间神经元的位置。攀爬纤维输入通过谷氨酸溢出激活许多中间神经元，但导致抑制接受攀爬纤维输入的相同浦肯野细胞的那些中间神经元的抑制，形成去抑制基序。这些中间神经元回路在感觉处理过程中参与，产生不同的路径特异性反应功能。这些发现表明，单个中间神经元对浦肯野细胞输出的强大影响可以通过各种中间神经元电路图案来塑造，以使小脑计算多样化。
The functional impact of single interneurons on neuronal output in vivo and how interneurons are recruited by physiological activity patterns remain poorly understood. In the cerebellar cortex, molecular layer interneurons and their targets, Purkinje cells, receive excitatory inputs from granule cells and climbing fibers. Using dual patch-clamp recordings from interneurons and Purkinje cells in vivo, we probe the spatiotemporal interactions between these circuit elements. We show that single interneuron spikes can potently inhibit Purkinje cell output, depending on interneuron location. Climbing fiber input activates many interneurons via glutamate spillover but results in inhibition of those interneurons that inhibit the same Purkinje cell receiving the climbing fiber input, forming a disinhibitory motif. These interneuron circuits are engaged during sensory processing, creating diverse pathway-specific response functions. These findings demonstrate how the powerful effect of single interneurons on Purkinje cell output can be sculpted by various interneuron circuit motifs to diversify cerebellar computations.