Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates
Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates
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DOI：
10.1177/002215549904700910
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发表时间：
1999-09-01
影响因子：
3.2
通讯作者：
Haugland, RP
中科院分区：
文献类型：
--
作者：
Panchuk-Voloshina, N;Haugland, RP;Haugland, RP
Alexa 350、Alexa 430、Alexa 488、Alexa 532、Alexa 546、Alexa 568和Alexa 594是一系列新的荧光染料，其发射/激发光谱分别与AMCA、荧光素黄、荧光素、罗丹明6G、四甲基罗丹明或Cy3、利沙明罗丹明B和德克萨斯红相似(Alexa名称中的数字表示近似激发波长最大值，单位为nm)。所有Alexa染料及其偶联物都比上面列出的常用光谱类似物更具荧光和光稳定性。此外，Alexa染料对pH值在4-10范围内不敏感。我们评价了Alexa染料与传统染料在应用中的不同应用，包括山羊抗鼠免疫球蛋白(GAM)、链霉亲和素、小麦胚凝集素(WGA)和刀豆蛋白A(ConA)。Alexa 546的偶联物比Cy3偶联物至少多两倍的荧光。用Alexa 568或Alexa 594染料标记的蛋白质比分别用李萨明罗丹明B或德克萨斯红染料标记的相同蛋白质亮几倍。Alexa染料衍生物的鬼臼蛋白染色F-肌动蛋白具有很高的特异性。Alexa染料的酰肼形式是非常明亮的、甲醛固定的极性示踪剂。Alexa 430(ex 430 nm/em 520 nm)和Alexa 532(ex 530 nm/em 548 nm)的共轭荧光是光谱上独特的荧光探针，在其激发和发射波长范围内具有相对较高的量子产率。
Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra similar to those of AMCA, Lucifer Yellow, fluorescein, rhodamine 6G, tetramethylrhodamine or Cy3, lissamine rhodamine B, and Texas Red, respectively (the numbers in the Alexa names indicate the approximate excitation wavelength maximum in nm). All Alexa dyes and their conjugates are more fluorescent and more photostable than their commonly used spectral analogues listed above. In addition, Alexa dyes are insensitive to pH in the 4-10 range. We evaluated Alexa dyes compared with conventional dyes in applications using various conjugates, including those of goat anti-mouse IgG (GAM), streptavidin, wheat germ agglutinin (WGA), and concanavalin A (ConA). Conjugates of Alexa 546 are at least twofold more fluorescent than Cy3 conjugates. Proteins labeled with the Alexa 568 or Alexa 594 dyes are several-fold brighter than the same proteins labeled with lissamine rhodamine B or Texas Red dyes, respectively. Alexa dye derivatives of phalloidin stain F-actin with high specificity. Hydrazide forms of the Alexa dyes are very bright, formaldehyde-fixable polar tracers. Conjugates of the Alexa 430 (ex 430 nm/em 520 nm) and Alexa 532 (ex 530 nm/em 548 nm) fluorochromes are spectrally unique fluorescent probes, with relatively high quantum yields in their excitation and emission wavelength ranges.