High density DNA methylation array with single CpG site resolution
High density DNA methylation array with single CpG site resolution
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DOI：
10.1016/j.ygeno.2011.07.007
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发表时间：
2011-10-01
期刊：
影响因子：
4.4
通讯作者：
Shen, Richard
中科院分区：
文献类型：
--
作者：
Bibikova, Marina;Barnes, Bret;Shen, Richard
我们已经开发了新一代全基因组DNA甲基化微珠芯片，它可以高通量地对人类基因组进行甲基化分析。新的高密度珠芯片可以分析超过480K的CpG位点，并并行分析12个样品。创新的内容包括覆盖99%的RefSeq基因，每个基因有多个探针，来自UCSC数据库的96%的CpG岛，CpG岛海岸，以及从全基因组亚硫酸氢盐测序数据和DNA甲基化专家输入的额外内容。功能完善的Infinium(R)分析用于使用亚硫酸氢盐转化的基因组DNA进行CpG甲基化分析。我们应用这项技术分析了正常和肿瘤DNA样本中的DNA甲基化，并将结果与相同样本的全基因组亚硫酸氢盐测序(WGBS)数据进行了比较。通过阵列和测序方法生成高度可比性的DNA甲基化图谱(平均R-2为0.95)。确定全基因组甲基化模式的能力将迅速推进甲基化研究。(C)2011 Elsevier Inc.保留所有权利。
We have developed a new generation of genome-wide DNA methylation Bead Chip which allows high-throughput methylation profiling of the human genome. The new high density BeadChip can assay over 480K CpG sites and analyze twelve samples in parallel. The innovative content includes coverage of 99% of RefSeq genes with multiple probes per gene, 96% of CpG islands from the UCSC database, CpG island shores and additional content selected from whole-genome bisulfite sequencing data and input from DNA methylation experts. The well-characterized Infinium (R) Assay is used for analysis of CpG methylation using bisulfite-converted genomic DNA. We applied this technology to analyze DNA methylation in normal and tumor DNA samples and compared results with whole-genome bisulfite sequencing (WGBS) data obtained for the same samples. Highly comparable DNA methylation profiles were generated by the array and sequencing methods (average R-2 of 0.95). The ability to determine genome-wide methylation patterns will rapidly advance methylation research. (C) 2011 Elsevier Inc. All rights reserved.