Reproductive Immunology
Reproductive Immunology
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DOI：
10.1036/1097-8542.581460
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发表时间：
2020-02
期刊：
影响因子：
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通讯作者：
Mariana Morena;Vieira Santos-Greatti;Márcia Guimarães da Silva;Carolina Sanitá;Tafner Ferreira;C. Marconi
中科院分区：
文献类型：
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作者：
Mariana Morena;Vieira Santos-Greatti;Márcia Guimarães da Silva;Carolina Sanitá;Tafner Ferreira;C. Marconi
研究表明，不仅细菌性阴道病，而且阴道中间菌群也会对女性的生殖健康产生有害影响。然而，文献仍然缺乏有关中间菌群的微生物学和免疫学方面的信息。目的：表征白细胞介素 (IL)–1β、IL-6、IL-8、肿瘤坏死因子-α、白细胞介素 1 受体拮抗剂 (IL-1ra)、IL-10、唾液酸酶水平的中间菌群；阴道加德纳菌负荷、细菌总数，并验证其是否与正常菌群或细菌性阴道病更密切相关。这项横断面研究纳入了 526 名未怀孕的育龄妇女，根据正常、中间和细菌性阴道病的 Nugent 系统的阴道菌群模式分为 3 组。分别通过 ELISA、MUAN 转换和定量实时 PCR 评估宫颈阴道细胞因子、唾液酸酶、阴道加泰罗尼亚负载和总细菌的水平。使用所有测量参数进行主成分分析（PCA）来比较三种不同类型的菌群。结果表明，与正常菌群相比，中间菌群与宫颈阴道IL-1β增加有关（P < 0.0001）。与细菌性阴道病相比，中间菌群的 IL-8 和 IL-10 水平较高（P < 0.01）。正常菌群和中间菌群中唾液酸酶的水平显着低于细菌性阴道病患者（P < 0.0001）。各组间阴道加拉特氏菌和细菌总数存在差异（P < 0.0001），其中细菌性阴道病最高。 PCA 显示正常菌群和中间菌群紧密分散，而细菌性阴道病则分开分组。结论：虽然中间菌群在细胞因子、唾液酸酶和细菌载量方面与正常菌群和细菌性阴道病有关，但综合考虑，中间菌群的一般微生物学和免疫学模式与正常菌群相似。
Studies have shown that not only bacterial vaginosis, but also intermediate vaginal flora has deleterious effects for women’s reproductive health. However, literature still lacks information about microbiological and immunological aspects of intermediate flora. Objective: To characterize intermediate flora regarding levels of Interleukin (IL)–1beta, IL-6, IL-8, tumor necrosis factor-alpha, interleukin 1 receptor antagonist (IL-1ra), IL-10, sialidase; loads of Gardnerella vaginalis , total bacteria and to verify whether it is closer related to normal flora or bacterial vaginosis. This cross-sectional study enrolled 526 non-pregnant reproductive-aged women distributed in 3 groups according to pattern of vaginal flora using Nugent’s system in normal, intermediate and bacterial vaginosis. Cervicovaginal levels of cytokines, sialidases, loads of G. vaginalis and total bacteria were assessed by ELISA, conversion of MUAN and quantitative real-time PCR, respectively. A principal component analysis(PCA) using all measured parameters was performed to compare the three different types of flora. Results showed that intermediate flora is associated with increased cervicovaginal IL-1beta in relation to normal flora(P < 0.0001). When compared to bacterial vaginosis, intermediate flora has higher IL-8 and IL-10 levels(P < 0.01). Sialidases were in sig-nificantly lower levels in normal and intermediate flora than bacterial vaginosis(P < 0.0001). Loads of G. vaginalis and total bacterial differed among all groups(P < 0.0001), being highest in bacterial vaginosis. PCA showed that normal and intermediate flora were closely scattered, while bacterial vaginosis were grouped separately. Conclusion: Although intermediate flora shows some differences in cytokines, siali-dases and bacterial loads in relation to normal flora and bacterial vaginosis, when taken together, general microbiological and immunological pattern pattern of intermediate flora resembles the normal flora.