Expression and clinical value of peroxiredoxin-1 in patients with pancreatic cancer
Expression and clinical value of peroxiredoxin-1 in patients with pancreatic cancer
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Peroxiredoxin-1在胰腺癌中的表达及临床价值
DOI：
10.1016/j.ejso.2014.11.037
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发表时间：
2015-02-01
期刊：
影响因子：
3.8
通讯作者：
Tang, Z. -G.
中科院分区：
文献类型：
--
作者：
Cai, C. -Y.;Zhai, L. -L.;Tang, Z. -G.
背景：过氧化物酶-1（Peroxiredoxin-1，Prx-1）是氧化还原损伤的重要保护因子，其在多种肿瘤中的异常表达不断被报道。本研究旨在探讨Prx-1在胰腺癌组织中的表达情况，并探讨其临床意义。方法：采用免疫组化法检测Prx-1在胰腺癌组织及癌旁组织中的表达。结果：免疫组化结果显示Prx-1在胰腺癌组织中的阳性表达率（74.4%）明显高于癌旁组织（37.2%）（P < 0.05）。癌组织中Prx-1表达与血管内皮生长因子（VEGF）、微血管密度（MVD）呈正相关。ELISA结果显示，胰腺癌患者的血清Prx-1水平高于健康受试者（31.2 +/- 13.5 vs. 13.2 +/- 11.9 ng/ml，p < 0.001）。Prx-1表达与临床病理参数的侵袭性相关。血清Prx-1与CA 19 -9联合检测，其曲线下面积（AUC）明显高于Prx-1单独检测。Prx-1阳性表达与令人失望的总生存期（OS）（p = 0.002）和无病生存期（DFS）（p <0.001）相关。多因素分析显示Prx-1是胰腺癌患者生存率（p = 0.035）和无病生存率（p <0.001）的独立生物标志物。Prx-1表达上调与肿瘤血管生成密切相关，是胰腺癌诊断和预后的重要肿瘤标志物。(C)2014爱思唯尔有限公司版权所有。
Background: Peroxiredoxin-1 (Prx-1) is an important protector for redox damage and its abnormal expression is continually reported in various tumors. This study aims to investigate the expression status of Prx-1 and evaluate its clinical value in pancreatic cancer.Methodology: Immunohistochemistry was used to detect Prx-1 expression in pancreatic cancer tissues and para-cancerous tissues. Enzyme-inked immunosorbent assay (ELISA) method was applied to detect the serum Prx-1 levels.Results: The immunohistochemical results indicated that positive rate of Prx-1 was (p < 0.05) higher in pancreatic cancer tissues (74.4%) than in para-cancerous tissues (37.2%). Prx-1 expression was positively correlated with vascular endothelial growth factor (VEGF) and microvessel density (MVD) in cancer tissues. The ELISA results showed that patients with pancreatic cancer had a higher serum Prx-1 level than healthy subjects (31.2 +/- 13.5 vs. 13.2 +/- 11.9 ng/ml, p < 0.001). Prx-1 expression was correlated with aggressive clinicopathological parameter. The combination of serum Prx-1 and CA19-9, the area under the curve (AUC) was significantly higher than Prx-1 separate. Positive Prx-1 expression was correlated with disappointing overall survival (OS) (p = 0.002) and disease-free survival (DFS) (p < .001). Multivariate analysis showed that Prx-1 staining as an independent biomarker of poor OS (p = 0.035) and DFS (p < .001).Conclusion: These findings suggest that the levels of Prx-1 expression are significantly increased in pancreatic cancer. The up-regulated Prx-1 is closely related to tumor angiogenesis and acts as a promising tumor marker for diagnosis and prognosis of pancreatic cancer. (C) 2014 Elsevier Ltd. All rights reserved.