Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
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DOI：
10.1590/s0102-09352007000300016
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发表时间：
2007-06-01
期刊：
影响因子：
--
通讯作者：
Kotait, I.
中科院分区：
文献类型：
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作者：
Castilho, J.G.;Brandão, P.E.;Kotait, I.
采用RT-PCR扩增了犬瘟热病毒（CDV） N基因480bp片段，结果发现2000 - 2004年间采集的11份流浪狗中枢神经系统样本呈阳性。基于部分n基因序列的系统发育分析显示出4个主要聚类。所有犬株分离为聚类1，与Onderstepoort和Lederle疫苗株的平均核苷酸同源性分别为95.8%和95.6%。聚类11包含所有浣熊相关毒株，聚类III为Orient毒株，聚类IV为Onderstepoort和Lederle疫苗毒株，它们之间的平均核苷酸同源性为99.7%。这是巴西首次报道CDV毒株的系统发育分析。
Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster 1, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster 11 contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.