Neuronal properties in cultured ultimobranchial C cells of chick embryos: process outgrowth and expression of TuJ1 and enkephalin
Neuronal properties in cultured ultimobranchial C cells of chick embryos: process outgrowth and expression of TuJ1 and enkephalin
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鸡胚培养的上鳃 C 细胞的神经元特性：TuJ1 和脑啡肽的生长和表达过程
DOI：
10.1016/s0006-8993(01)02038-8
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发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Y. Kameda
中科院分区：
文献类型：
--
作者：
M. Miura;Y. Kameda
我们通过免疫组织化学和电子显微镜分析了从不同发育阶段（孵化 12-16 天）的胚胎鸡中分离出的培养的上鳃 C 细胞的神经元特性。上鳃腺主要由 C 细胞固体组成。在 13 天大的胚胎中，许多 C 细胞在层粘连蛋白包被的载玻片上培养 7 天，延长了长的神经突样突起，长度达到 300 μm。 C 细胞的神经炎生长受到发育调节，并且几乎不受神经生长因子 (NGF) 的影响。培养的 C 细胞对降钙素和脑啡肽表现出强烈的免疫反应性。共聚焦激光扫描显微镜还证实，几乎所有 C 细胞都被降钙素抗血清和单克隆抗体 TuJ1（一种神经元特异性标记物）强烈免疫染色。扫描电子显微镜发现 C 细胞胞体中长有分支的神经突。这个过程沿途有许多曲折，并以生长锥结束。具有突起的 C 细胞通常是单极的，较少见的是双极或多极的。透射电子显微镜显示培养的 C 细胞中存在膜结合的分泌颗粒。 C细胞的神经突包含平行于长轴排列的微丝、中间丝和微管的聚集体。此外，在突触末端和C细胞表面之间形成突触样接触，显示桥粒样膜增厚以及小的透明囊泡和致密核囊泡的积累。这些结果表明，从早期鸡胚（12 日龄和 13 日龄）培养的 C 细胞在体外长期保持神经元特征。
We have analyzed the neuronal properties in cultured ultimobranchial C cells isolated from embryonic chicks at different developmental stages (12–16 days of incubation) by immunohistochemistry and electron microscopy. The ultimobranchial glands mostly consist of C cell solids. In 13-day-old embryos, many C cells cultured for 7 days on laminin-coated slides extended long neurite-like processes, reaching 300 μm in length. Neuritic outgrowth of C cells was regulated developmentally and virtually unaffected by nerve growth factor (NGF). The cultured C cells expressed intense immunoreactivity for calcitonin and enkephalin. It was also confirmed by confocal laser-scanning microscopy that almost all C cells were intensely immunostained by both the calcitonin antiserum and the monoclonal antibody TuJ1, a neuron-specific marker. Scanning electron microscopy identified the outgrowth of long, branching neuritic processes emerging from C cell soma. The processes had numerous varicosities along their course and ended in growth cones. The C cells with processes were usually monopolar and less frequently bipolar or multipolar. Transmission electron microscopy revealed the presence of membrane-bounded secretory granules in the cultured C cells. The neuritic processes of C cells contained aggregations of microfilaments, intermediate filaments and microtubules arranged in parallel to the long axis. In addition, synaptic-like contacts showing desmosome-like membrane-thickenings and accumulations of small clear vesicles and dense-cored vesicles were formed between the endings of the processes and the surface of C cells. These results indicate that the C cells cultured from early chick embryos (12- and 13-day-old) maintain the neuronal characteristics for long periods in vitro.
产生神经降压素和降钙素的大鼠甲状腺髓样癌细胞克隆株。
DOI：
--
发表时间：
1983
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Zeytinoglu,FN;Gagel,RF;DeLellis,RA;Wolfe,HJ;TashjianJr,AH;Hammer,RA;Leeman,SE
通讯作者：
Leeman,SE
影响因子：
--
作者：
LEE, MK;TUTTLE, JB;FRANKFURTER, A
通讯作者：
FRANKFURTER, A
甲状腺 C 细胞系的神经元特性：地塞米松和视黄酸的部分抑制。
DOI：
10.1210/mend.6.2.1569964
发表时间：
1992
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Russo,AF;Lanigan,TM;Sullivan,BE
通讯作者：
Sullivan,BE