Encapsulation of phycocyanin by prebiotics and polysaccharides-based electrospun fibers and improved colon cancer prevention effects.
Encapsulation of phycocyanin by prebiotics and polysaccharides-based electrospun fibers and improved colon cancer prevention effects.
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DOI：
10.1016/j.ijbiomac.2020.01.189
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发表时间：
2020-01
影响因子：
8.2
通讯作者：
Yan Wen;Peng Wen;Teng-gen Hu;R. Linhardt;Min-hua Zong;Hong Wu;Zhi-Yi Chen
中科院分区：
文献类型：
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作者：
Yan Wen;Peng Wen;Teng-gen Hu;R. Linhardt;Min-hua Zong;Hong Wu;Zhi-Yi Chen
为保持藻蓝蛋白（PC）的生物活性并实现其结肠靶向释放，制备了含有PC和益生元的多糖基静电纺丝纤维毡（EFM），并对其进行了表征。体外释放实验证实了PC的结肠靶向行为，特别是由于益生元的加入，PC实现了更快的释放。Ritger-Peppas模型证实PC在模拟结肠液中的释放遵循异常转运机制（非Fickian）。CCK-8结果显示，PC和益生元组合对HCT 116细胞具有显著的抗增殖作用，在24、48和72 h后的IC 50值分别为22.31、17.12和11.63 mg/mL。细胞周期和凋亡分析表明，该化合物对HCT 116细胞的抑制作用是通过抑制细胞周期蛋白D1（cyclin D1）和细胞周期蛋白依赖性激酶4（CDK 4）的表达，上调p21蛋白的表达，使细胞周期阻滞于G 0/G1期，并通过介导线粒体途径诱导细胞凋亡，其中Bcl-2/Bax比值降低，包括caspase 3的激活和细胞色素c的释放。这项研究表明，PC负载的EFM与GOS具有很大的潜力，作为一种有效的结肠癌预防制剂。
To preserve bioactivity and achieve colon targeted release of phycocyanin (PC), the polysaccharides-based electrospun fiber mat (EFM) containing PC and prebiotics was prepared and characterized. In vitro release tests confirmed the colon targeting behavior of PC, in particular, faster release of PC was achieved due to the addition of prebiotics. Ritger–Peppas model confirmed that the release of PC in simulated colon fluids follows a mechanism of anomalous transport (non-Fickian). CCK-8 results showed that the combination of PC and prebiotics exerted a significant anti-proliferative effect on HCT116 cells with an IC50 values of 22.31, 17.12 and 11.63 mg/mL after 24, 48, and 72 h, respectively. Furthermore, the cell cycle and apoptosis analysis revealed that the inhibition activity on HCT116 cells was caused by arresting cell cycle at G0/G1 phase that is relevant to the inhibition of cyclin D1 and CDK4 and the up-regulation of p21 expression, and inducing cell apoptosis by mediating the mitochondrial pathway as well, in which the decrease of Bcl-2/Bax, activation of caspase 3 and release of cytochromecwere included. This study suggests that the PC-loaded EFM with GOS holds a great potential as an effective formulation for colon cancer prevention.