Targeted pH switched europium complexes monitoring receptor internalisation in living cells.
Targeted pH switched europium complexes monitoring receptor internalisation in living cells.
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DOI：
10.1039/d1cc01029h
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发表时间：
2021-05
影响因子：
4.9
通讯作者：
Jack D. Fradgley;Matthieu Starck;M. Laget;Emmanuel Bourrier;Elodie Dupuis;L. Lamarque;E. Trinquet;J. Zwier;D. Parker
中科院分区：
文献类型：
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作者：
Jack D. Fradgley;Matthieu Starck;M. Laget;Emmanuel Bourrier;Elodie Dupuis;L. Lamarque;E. Trinquet;J. Zwier;D. Parker
我们报道了pH响应发光铕（iii）探针的设计和评估，该探针允许偶联靶向载体以监测细胞中的受体内化。本文采用的方法可用于选择性地标记蛋白质并监测更多酸性细胞器的摄取，从而提高需要实时监测pH值的时间分辨内化分析的性能。
We report the design and evaluation of pH responsive luminescent europium(iii) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.