Brn-3a is required for the generation of proprioceptors in the mesencephalic trigeminal tract nucleus.
Brn-3a is required for the generation of proprioceptors in the mesencephalic trigeminal tract nucleus.
复制标题
Brn-3a 是中脑三叉神经束核中本体感受器生成所必需的。
DOI：
10.1016/j.brainres.2005.06.026
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Sugimoto,T
中科院分区：
文献类型：
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作者：
Ichikawa,H;Qiu,F;Xiang,M;Sugimoto,T
在胚胎第 18.5 天和出生后第 0 天，研究了野生型和 Brn-3a 敲除小鼠三叉神经系统中运动神经元和本体感觉神经元的分布。我们发现，野生型（每节平均数±SD = 128 ± 22，范围= 93–167）和敲除型（每节平均数± SD）的三叉神经运动核（Mo5）含有丰富的运动神经元。切片 = 121 ± 23，范围 = 75–158）小鼠，并且这些小鼠之间 Mo5 神经元的细胞大小相似（野生型，平均值 ± SD = 165 ± 59 μm2，范围 = 65–326 μm2；敲除，平均值 ± SD = 167 ± 59 μm2，范围 = 71–327 μm2）。 Mo5神经元对降钙素基因相关肽具有免疫反应性，并且这种免疫反应性神经元在野生型和突变型小鼠中都很丰富。在野生型小鼠的中脑束核 (Mes5) 中，还观察到许多含有小清蛋白免疫反应性的本体感受器（每节平均数 ± SD = 56 ± 19，范围 = 27–85）。然而，在基因敲除小鼠中，无法检测到 Mes5 神经元。通常含有 Mes5 的脑干区域缺乏小清蛋白免疫反应性本体感受器。本研究表明，Brn-3a 是三叉神经系统本体感受器发育所必需的，但运动神经元则不然。
The distribution of motor and proprioceptive neurons was investigated in the trigeminal nervous system of wild-type and Brn-3a knockout mice at embryonic day 18.5 and postnatal day 0. We found that the trigeminal motor nucleus (Mo5) contained abundant motoneurons in wild-type (mean number ± SD per section = 128 ± 22, range = 93–167) and knockout (mean number ± SD per section = 121 ± 23, range = 75–158) mice and that the cell size of Mo5 neurons was similar between these mice (wild-type, mean ± SD = 165 ± 59 μm2, range = 65–326 μm2; knockout, mean ± SD = 167 ± 59 μm2, range = 71–327 μm2). Mo5 neurons were immunoreactive for calcitonin gene-related peptide and such immunoreactive neurons were abundant in both wild-type and mutant mice. In the mesencephalic tract nucleus (Mes5) of wild-type mice, many proprioceptors (mean number ± SD per section = 56 ± 19, range = 27–85) that contained parvalbumin immunoreactivity were also observed. In knockout mice, however, Mes5 neurons could not be detected. The area of brainstems which normally contained the Mes5 was devoid of parvalbumin-immunoreactive proprioceptors. The present study suggests that Brn-3a is required for the development of proprioceptors but not motoneurons in the trigeminal nervous system.