Genome Mining and Metabolomics Uncover a Rare d-Capreomycidine Containing Natural Product and Its Biosynthetic Gene Cluster.
Genome Mining and Metabolomics Uncover a Rare d-Capreomycidine Containing Natural Product and Its Biosynthetic Gene Cluster.
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DOI：
10.1021/acschembio.0c00663
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发表时间：
2020-11-20
影响因子：
4
通讯作者：
Thomson RJ
中科院分区：
文献类型：
--
作者：
Tryon JH;Rote JC;Chen L;Robey MT;Vega MM;Phua WC;Metcalf WW;Ju KS;Kelleher NL;Thomson RJ
We report the metabolomics-driven genome mining of a new cyclic-guanidino incorporating non-ribosomal peptide synthetase (NRPS) gene cluster and full structure elucidation of its associated hexapeptide product, faulknamycin. Structural studies unveiled that this natural product contained the previously unknown (R,S)-stereoisomer of capreomycidine, d-capreomycidine. Furthermore, heterologous expression of the identified gene cluster successfully reproduces faulknamycin production without an observed homologue of VioD, the pyridoxal phosphate (PLP)-dependent enzyme found in all previous l-capreomycidine biosynthesis. An alternative NRPS-dependent pathway for d-capreomycidine biosynthesis is proposed.
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影响因子：
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作者：
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通讯作者：
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