Genomic Analysis of the Principal Members of Antioxidant Enzymes in Simulated Stresses Response and Postharvest Physiological Deterioration in Cassava
Genomic Analysis of the Principal Members of Antioxidant Enzymes in Simulated Stresses Response and Postharvest Physiological Deterioration in Cassava
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DOI：
10.1007/s12042-021-09304-4
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发表时间：
2021-10
影响因子：
2
通讯作者：
Sang Shang;Yuqi Tang;Jing Dai;Chunlai Wu;Yan Yan-Yan;Weiwei Tie;Meiying Li;Jinghao Yang;Jian Zeng;Ming-jie Chen;Wei Hu
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文献类型：
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作者：
Sang Shang;Yuqi Tang;Jing Dai;Chunlai Wu;Yan Yan-Yan;Weiwei Tie;Meiying Li;Jinghao Yang;Jian Zeng;Ming-jie Chen;Wei Hu
活性氧是生物生长发育过程中的一种信号分子。活性氧的稳态需要不同的抗氧化防御机制来维持。目前，木薯中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）作为主要的抗氧化酶还不清楚。本研究利用隐马尔可夫模型从木薯基因组中鉴定出7个sod、6个cat和6个apx，并通过基因结构、蛋白基序和系统发育关系分析得到支持。SOD、CAT和apx基因在木薯不同组织中的表达存在差异，其中大部分SOD表达量较高。综合表达谱显示，sod、CAT、apx基因参与贮藏根采后生理变质（PPD）过程以及对渗透胁迫和ABA的响应。总之，本研究增加了我们对木薯PPD过程中、生物胁迫和非生物胁迫下木薯od、CAT、apx基因特征及其潜在功能的认识，为进一步分析木薯基因功能奠定了坚实的基础。
ROS act as an signaling molecule in the biological growth and development process. The homeostasis of ROS must be kept by different antioxidant defense mechanisms. Currently, superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) as major antioxidant enzymes are not well understood in cassava (Manihot esculenta). In this research, 7 SODs, 6 CATs, and 6 APXs were identified from the cassava genome by hidden Markov models, which was supported by gene structure, protein motifs, and phylogenetic relationship analyses.SOD, CAT,andAPXgenes expressed differentially in different tissues of cassava, of which mostSODsshowed high expression levels. The comprehensive expression profiles revealed the participation ofSOD,CAT,APXgenes during postharvest physiological deterioration (PPD) of storage root and in response to osmotic stress and ABA as well asXanthomonas axonopodisinfection. Together, this study increases our understanding of cassavaSOD,CAT,APXgenes feature and their potential function during PPD process and in response to biotic and abiotic stresses in cassava, laying a solid foundation for further gene function analysis in cassava.