Correlations of structure and rates of energy transfer for through-bond energy-transfer cassettes
Correlations of structure and rates of energy transfer for through-bond energy-transfer cassettes
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DOI：
10.1021/jp053388z
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发表时间：
2006-01-12
影响因子：
2.9
通讯作者：
Topp, MR
中科院分区：
文献类型：
--
作者：
Kim, TG;Castro, JC;Topp, MR
荧光DNA标记盒被设计成具有与单个激发激光波长匹配的共同吸收发色团，但最多有四种不同的发射体。这里报道的实验已经检查了两种不同类型的盒的能量转移速率和荧光偏振特性，涉及三个不同的相对取向的供体和受体的过渡时刻和刚性接头的轴。能量转移时间的范围从< 200 fs到近似20 ps，当供体和受体物质的跃迁时刻平行于连接体轴排列时发生最快的转移时间。实验证据支持通过键能量转移机制，与商业DNA标记剂，表现出慢得多的转移时间控制FRET相反。这些刚性盒也表现出来自受体物质的偏振荧光，因此这种特定类型的DNA标记探针具有单分子探针如罗丹明和香豆素染料的一些优点。
Fluorescent DNA-labeling cassettes are designed to have a common absorbing chromophore matched to a single exciting laser wavelength, but up to four different emitters. Experiments reported here have examined the energy-transfer rates and fluorescence polarization characteristics for two different types of cassette, involving three distinct relative orientations of the donor and acceptor transition moments and the axis of the rigid linker. Energy-transfer times range from < 200 fs to similar to 20 ps, the fastest transfer times occurring when the transition moments of the donor and acceptor species are aligned parallel to the linker axis. Experimental evidence is presented that supports a through-bond energy-transfer mechanism, in contrast with a commercial DNA-labeling agent, which exhibits much slower transfer times controlled by FRET. These rigid cassettes also exhibit polarized fluorescence from the acceptor species, so that this particular type of DNA-labeling probe has some of the advantages of single-molecule probes such as rhodamine and coumarin dyes.