Characterization and Phylogenetic Analysis of the Complete Mitochondrial Genome of Laelia suffusa (Lepidoptera: Erebidae, Lymantriinae).
Characterization and Phylogenetic Analysis of the Complete Mitochondrial Genome of Laelia suffusa (Lepidoptera: Erebidae, Lymantriinae).
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Laelia suffusa（鳞翅目：Erebidae、Lymantriinae）完整线粒体基因组的表征和系统发育分析
DOI：
10.1093/jisesa/ieaa138
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发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Zhang AB
中科院分区：
文献类型：
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作者：
Li J;Lv Q;Zhang XM;Han HL;Zhang AB
摘要本研究对白色柞蚕（Lepidoptera: Erebidae, Lymantriinae） Laelia suffusa （Walker, 1855）的线粒体全基因组进行了测序和注释。该基因组序列全长15502 bp，由13个PCGs、2个rnas、22个trna和1个非编码控制区（CR）组成。基因组的核苷酸组成具有高度的A + T偏倚，占整个基因组的79.04%，AT偏度略为正（0.015）。与鳞翅目基种的基因序列比较，发现了典型的trnM重排。此外，trnM重排出现在trnI和trnQ的头部，而不是后部。除cox1使用CGA外，其余13个PCGs均使用ATN作为起始密码子。在22个tRNA中，只有1个tRNA （trnS1）在典型的三叶草二级结构中不能折叠。在控制区起始处检测到保守基序“ATAGA + poly-T”，与其他鳞翅目相似。共鉴定出10个重叠区和19个基因间间隔区，范围分别为1 ~ 41 bp和2 ~ 73 bp。系统发育分析表明，Lymantriinae属单系类群，支持度高，与Lymantriinae族Orgyiini （Erebidae, Lymantriinae）亲缘关系密切。利用13个PCGs和37个基因重构了夜蛾科（鳞翅目）物种的系统发育关系，支持了（Notodontidae + (Erebidae + (Nolidae + (Euteliidae + Noctuidae)))）)的拓扑结构。
Abstract In this study, the complete mitochondrial genome of a white tussock moth, Laelia suffusa (Walker, 1855) (Lepidoptera: Erebidae, Lymantriinae), was sequenced and annotated. The genome sequence was 15,502 bp in length and comprised 13 PCGs, 2 rRNAs, 22 tRNAs, and a single noncoding control region (CR). The nucleotide composition of the genome was highly A + T biased, accounting for 79.04% of the whole genome and with a slightly positive AT skewness (0.015). Comparing the gene order with the basal species of Lepidoptera, a typical trnM rearrangement was detected in the mitogenome of L. suffusa. Besides, the trnM rearrangement was found at the head of trnI and trnQ, rather than at the back. The 13 PCGs used ATN as their start codons, except for the cox1 which used CGA. Out of the 22 tRNAs, only 1 tRNA (trnS1) failed to fold in a typical cloverleaf secondary structure. The conserved motif ‘ATAGA + poly-T’ was detected at the start of the control region which was similar to other Lepidoptera species. In total, 10 overlapping regions and 19 intergenic spacers were identified, ranging from 1 to 41 and 2 to 73 bp, respectively. Phylogenetic analysis showed that Lymantriinae was a monophyletic group with a high support value and L. suffusa was closely related to tribe Orgyiini (Erebidae, Lymantriinae). Moreover, the phylogenetic relationship of Noctuoidea (Lepidoptera) species was reconstructed using two datasets (13 PCGs and 37 genes) and these supported the topology of (Notodontidae + (Erebidae + (Nolidae + (Euteliidae + Noctuidae)))).