Simplified colorimetric determination of thiocyanate in biological fluids, and its application to investigation of the toxic amblyopias.
Simplified colorimetric determination of thiocyanate in biological fluids, and its application to investigation of the toxic amblyopias.
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DOI：
10.1093/clinchem/18.9.996
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发表时间：
1972-09
影响因子：
9.3
通讯作者：
A. Pettigrew;G. Fell
中科院分区：
文献类型：
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作者：
A. Pettigrew;G. Fell
一种测定微量氰化物和硫氰酸盐的比色法，包括由吡啶和芳香胺反应合成一种吡啶染料，已被简化为仅用于生物体液中硫氰酸盐的测定。在比色反应中用对苯二胺取代联苯胺，既提高了分析过程的精密度，又避免了致癌危险。这种方法已经被用来跟踪与戒烟相关的血浆硫氰酸盐的下降，以及随后在戒烟后的上升。它还被用于测量患有特殊毒性弱视-烟草弱视和Leber遗传性视神经萎缩-的患者的血浆和尿硫氰酸盐浓度，据信与氰化物中毒有关，并跟踪各种治疗方法带来的患者视力显著改善所伴随的硫氰酸盐浓度升高。
A colorimetric procedure for determination of small amounts of cyanide and thiocyanate, involving the synthesis of a pyridine dyestuff by the reaction of pyridine and an aromatic amine, has been simplified for the estimation of thiocyanate alone in biological fluids. Replacement of benzidine with p -phenylenediamine in the colorimetric reaction has both improved the precision of the analytical procedure and avoided a carcinogenic hazard. This method has been used to follow the decrease in plasma thiocyanate associated with abstinence from cigarette smoking, and its subsequent increase upon resumption. It has also been used to measure the plasma and urinary thiocyanate concentrations of patients suffering from the particular toxic amblyopias—tobacco amblyopia and Leber’s hereditary optic atrophy— believed to be associated with cyanide toxicity, and to follow the increased thiocyanate concentrations that accompany significant improvements in the patients’ vision brought about by various treatments.