Isolation and characterization of a glycine transport mutant in an established mammalian cell line, CHO(PEOT/1).
Isolation and characterization of a glycine transport mutant in an established mammalian cell line, CHO(PEOT/1).
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在已建立的哺乳动物细胞系 CHO(PEOT/1) 中分离和表征甘氨酸转运突变体。
DOI：
10.1007/bf01534492
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Adelberg,EA
中科院分区：
文献类型：
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作者：
Fairgrieve,M;Mullin,JM;Dantzig,AH;Slayman,CW;Adelberg,EA
CHO(PEOT/1)细胞对甘氨酸的摄取至少由三个系统介导，其中两个系统在本研究中已被鉴定和部分描述：(1)一个低亲和力的“A”系统，它运输许多小的中性氨基酸，包括甘氨酸和甲氨基异丁酸(MeAIB)；(2)一个高亲和力的系统，专为甘氨酸和肌氨酸所特有。通过氚自杀和复制培养的组合，我们分离到一株突变株(CHY-3)，在标准测试浓度2.5ΜM下，甘氨酸转运降低了47%。对放射性甘氨酸、MeAIB和肌氨酸的摄取研究表明，该突变株缺乏甘氨酸-肌氨酸系统，但在A系统经历了30-50%的代偿性增加。因此，在CHO细胞对甘氨酸的摄取方面，这两个系统之间似乎存在着调节的相互作用。
Glycine uptake in CHO(PEOT/1) cells is mediated by at least three systems, of which two have been identified and partially characterized in this study: (1) a low affinity “A” system that transports a number of small neutral amino acids including glycine and methylaminoisobutyric acid (MeAIB), and (2) a highaffinity system, specific for glycine and sarcosine. By a combination of tritium suicide and replica plating, we have isolated a mutant (CHY-3) with a 47% decrease in glycine transport at the standard test concentration of 2.5 ΜM. Uptake studies with radioactive glycine, MeAIB, and sarcosine revealed that the mutant lacks the glycine-sarcosine system, but has undergone a compensatory 30–50% increase in the A system. Thus, there appears to be a regulatory interaction between these two systems for glycine uptake by CHO cells.