Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values
Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values
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DOI：
10.1023/a:1008395916985
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发表时间：
1999-02-01
影响因子：
2.7
通讯作者：
Bax, A
中科院分区：
文献类型：
--
作者：
Ottiger, M;Bax, A
它表明，双十四烷基-磷脂酰胆碱或双十二烷基-磷脂酰胆碱和二己基-磷脂酰胆碱在水中的混合物形成溶致液晶相，在类似的条件下，如先前报道的双胞组成的二肉豆蔻酰-磷脂酰胆碱（DMPC）和二己酰-磷脂酰胆碱（DHPC）。DMPC和DHPC中存在的羧基-酯键被其烷基类似物中的醚键取代，这防止了这些化合物的酸或碱催化水解。在2.3-10.4 pH范围内测量的泛素的N-15-H-1偶极偶联表明，该蛋白质在整个范围内保持与其在pH 6.5时的结构非常相似的骨架构象。
It is demonstrated that mixtures of ditetradecyl-phosphatidylcholine or didodecyl-phoshatidylcholine and dihexyl-phosphatidylcholine in water form lyotropic liquid crystalline phases under similar conditions as previously reported for bicelles consisting of dimyristoyl-phosphatidylcholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC). The carboxy-ester bonds present in DMPC and DHPC are replaced by ether linkages in their alkyl analogs, which prevents acid- or base-catalyzed hydrolysis of these compounds. N-15-H-1 dipolar couplings measured for ubiquitin over the 2.3-10.4 pH range indicate that this protein retains a backbone conformation which is very similar to its structure at pH 6.5 over this entire range.