Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells.
Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells.
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DOI：
10.1155/2015/323246
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发表时间：
2015
影响因子：
--
通讯作者：
Lv J
中科院分区：
文献类型：
--
作者：
Ge H;Zhang F;Shan D;Chen H;Wang X;Ling C;Xi H;Huang J;Zhu C;Lv J
UCP2在多种组织中通过调节线粒体生物发生、维持能量平衡、消除ROS、调节细胞自噬等发挥生理作用。但UCP2在积云细胞中的确切作用尚不清楚。在培养基中加入一种特殊的UCP2抑制剂Genipin，探讨UCP2在人积云细胞中的作用。与对照组相比，UCP2抑制组积云细胞ATP和线粒体膜电位水平无显著差异。UCP2抑制Genipin后，ROS和Mn-SOD水平明显升高。然而，用Genipin治疗后，GSH减少与GSSG的比例显著下降。Genipin对UCP2的抑制也导致caspase-3活性明显升高，同时caspase-3 mRNA水平下降。经Genipin处理后，培养液中黄体酮水平明显下降。但雌二醇浓度无显著差异。本研究提示UCP2在人积云细胞中表达，在介导ROS生成、凋亡过程和甾体生成等方面发挥重要作用，提示UCP2可能参与卵泡发育和卵母细胞成熟及质量的调节。
UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues. But the exact roles of UCP2 in cumulus cells are still not clear. Genipin, a special UCP2 inhibitor, was added into the cultural medium to explore the roles of UCP2 in human cumulus cells. There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control. The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin. However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin. UCP2 inhibition by Genipin also resulted in obvious increase in the active caspase-3, which accompanied the decline of caspase-3 mRNA. The level of progesterone in culture medium declined obviously after Genipin treatment. But there was no significant difference in estradiol concentrations. This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality.