Localization of HtrA2/Omi immunoreactivity in brains affected by Alzheimer’s disease
Localization of HtrA2/Omi immunoreactivity in brains affected by Alzheimer’s disease
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DOI：
10.1097/wnr.0b013e328340a731
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发表时间：
2010-12
期刊：
影响因子：
1.7
通讯作者：
Y. Kawamoto;Hidefumi Ito;Yoshito Kobayashi;Yasuyuki Suzuki;R. Takahashi
中科院分区：
文献类型：
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作者：
Y. Kawamoto;Hidefumi Ito;Yoshito Kobayashi;Yasuyuki Suzuki;R. Takahashi
HtrA 2/Omi是一种促进凋亡过程的线粒体丝氨酸蛋白酶，本研究调查了HtrA 2/Omi的异常免疫表达是否发生在阿尔茨海默病患者中。我们从7名对照个体和7名阿尔茨海默病患者中制备了尸检大脑，然后使用福尔马林固定的石蜡包埋切片对所有这些病例进行了HtrA 2/Omi的免疫组织化学研究。在大脑皮层和海马的阿尔茨海默病的情况下，密集积累的HtrA 2/Omi免疫反应是分散的，细胞内和细胞外。双重免疫荧光分析显示HtrA 2/Omi免疫反应性在淀粉样肽免疫阳性老年斑和磷酸化免疫阳性神经元缠结中的部分定位。这些结果表明，HtrA 2/Omi可能与阿尔茨海默病的发病机制部分相关。
HtrA2/Omi is a mitochondrial serine protease that promotes apoptotic processes, and this study investigated whether the abnormal immunoexpression of HtrA2/Omi occurs in patients with Alzheimer’s disease. We prepared autopsied brains from seven control individuals and seven patients with Alzheimer’s disease, and then carried out immunohistochemical studies on HtrA2/Omi using formalin-fixed, paraffin-embedded sections from all of these cases. In the cerebral cortex and hippocampus from the cases of Alzheimer’s disease, densely accumulated HtrA2/Omi immunoreactivity was scattered, both intracellularly and extracellularly. Double immunofluorescence analyses showed the partial localization of HtrA2/Omi immunoreactivity in amyloid -peptide-immunopositive senile plaques and phosphorylated -immunopositive neurofibrillary tangles. These results suggest that HtrA2/Omi may be partially associated with the pathogenesis of Alzheimer’s disease.