In situ hybridization to chromosomes stabilized in gel microdrops.
In situ hybridization to chromosomes stabilized in gel microdrops.
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与稳定在凝胶微滴中的染色体进行原位杂交。
DOI：
10.1002/cyto.990210202
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Weier,HU
中科院分区：
文献类型：
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作者：
Nguyen,BT;Lazzari,K;Abebe,J;Mac,I;Lin,JB;Chang,A;Wydner,KL;Lawrence,JB;Cram,LS;Weier,HU
传统的染色体原位杂交方法依赖于固定在玻片上，然后进行显微镜评价。本报告描述了一种微滴原位杂交（MISH）方法的发展，该方法便于在悬浮中与染色体杂交。由琼脂糖基质组成的凝胶微滴（GMDs）包裹的染色体经受住了严格的杂交和变性条件。由于增加了稳定性，对被封装染色体的杂交可以通过流式细胞术和常规显微镜进行检测。因此，MISH方法提供了一种无需载玻片的染色体杂交方法，并且可以使用杂交而不是核酸结合染料来鉴定和分离染色体。©1995 Wiley‐Liss, Inc。
Conventional chromosome in situ hybridization procedures rely on fixation to glass slides followed by microscopic evaluation. This report describes the development of a microdrop in situ hybridization (MISH) method which facilitates hybridization to chromosomes in suspension. Chromosomes encapsulated in gel microdrops (GMDs) composed of an agarose matrix withstood stringent hybridization and denaturation conditions. Because of the increased stability, hybridization to encapsulated chromosomes was detected by flow cytometry as well as conventional microscopy. Thus, the MISH method offers a means for chromosome hybridization without slides and may enable identification and isolation of chromosomes using hybridization rather than nucleic acid binding dyes. © 1995 Wiley‐Liss, Inc.