Involvement of Populus CLEL peptides in root development
Involvement of Populus CLEL peptides in root development
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杨树 CLEL 肽参与根发育
DOI：
10.1093/treephys/tpz084
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发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Shi Xueping
中科院分区：
文献类型：
--
作者：
Tian Dongdong;Liu Yueyuan;Tian Lidan;Wan Mengjie;Zheng Bo;Shi Xueping
作为主要的小翻译后修饰肽之一，CLV3/胚胎周围区域相关（CLE）样（CLEL）肽家族已被报道可调节拟南芥的根生长、侧根发育和植物向重力反应。在本研究中，我们鉴定了毛果杨中的12个CLEL基因，并对这些基因进行了全面的生物信息学分析。其中，5P。用新的基因模型修改了毛果 CLEL（PtrCLEL）。所有这些 PtrCLEL 蛋白在结构上都与 A.拟南芥 CLELs(AtCLELs)，包括 N 端信号肽、保守的 C 端 13 个氨基酸 CLEL 基序和可变中间区。计算机模拟和实时定量 PCR 分析表明，PtrCLEL 在多种组织中广泛表达，包括根、叶、芽和茎。外源应用化学合成的 PtrCLEL 肽导致 A 中出现波状或卷曲的根并减少侧根形成。拟南芥。此外，在含有这些肽的生长培养基上发芽的三角杨幼苗会导致根增厚并形成异常的侧根，在许多情况下呈簇状。通过用 PtrCLEL10 肽处理 Populus717 插条，进一步研究了增厚根的解剖学和组织学变化。我们观察到根部增厚主要是由于表皮、皮下组织和皮质中细胞数量的增加。我们的研究结果表明，PtrCLE和AtCLEL基因编码的蛋白质具有相似的蛋白质结构、肽基序序列和发育根的肽活性。 PtrCLEL 肽在根发育中的活性具有物种依赖性。
As one of the major groups of small post-translationally modified peptides, the CLV3/EMBRYO SURROUNDING REGION-RELATED (CLE)-like (CLEL) peptide family has been reported to regulate root growth, lateral root development and plant gravitropic responses inArabidopsis thaliana. In this study, we identified 12CLELgenes inPopulus trichocarpaand performed a comprehensive bioinformatics analysis on these genes. Among them, fiveP. trichocarpa CLELs (PtrCLELs)were revised with new gene models. All of these PtrCLEL proteins were structurally similar to theA. thaliana CLELs(AtCLELs), including an N-terminal signal peptide, a conserved C-terminal 13-amino-acid CLEL motif and a variable intermediate region. In silico and quantitative real-time PCR analyses showed thatPtrCLELswere widely expressed in various tissues, including roots, leaves, buds and stems. Exogenous application of chemically synthesized PtrCLEL peptides resulted in wavy or curly roots and reduced lateral root formation inA. thaliana. Moreover, germinatingPopulus deltoidesseedlings on a growth medium containing these peptides caused the roots to thicken and to form abnormal lateral roots, in many cases in clusters. Anatomical and histological changes in thickened roots were further investigated by treatingPopulus717 cuttings with the PtrCLEL10 peptide. We observed that root thickening was mainly due to an increased number of cells in the epidermis, hypodermis and cortex. The results of our study suggested thatPtrCLELandAtCLELgenes encode proteins with similar protein structures, sequences of peptide motif and peptide activities on developing roots. The activities of PtrCLEL peptides in root development were species-dependent.