Genome Analysis of Staphylococcus capitis TE8 Reveals Repertoire of Antimicrobial Peptides and Adaptation Strategies for Growth on Human Skin
Genome Analysis of Staphylococcus capitis TE8 Reveals Repertoire of Antimicrobial Peptides and Adaptation Strategies for Growth on Human Skin
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DOI：
10.1038/s41598-017-11020-7
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发表时间：
2017-09-05
影响因子：
4.6
通讯作者：
Sharma, Rakesh
中科院分区：
文献类型：
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作者：
Kumar, Rohit;Jangir, Pramod Kumar;Sharma, Rakesh
从健康人足部皮肤表面分离得到一株头葡萄球菌（Staphylococcus capitis）TE 8，该菌株对革兰氏阳性菌（包括金黄色葡萄球菌）具有较强的抗菌活性。S.通过鸟枪法和双端焦磷酸测序获得了capitis TE 8，覆盖率为109倍。基因组草图在8个支架中含有2，516，639 bp，总共209个重叠群。基因组包含2319个蛋白质编码序列，58个tRNA和3个rRNA。基因组序列分析揭示了具有编码抗微生物肽能力的4个不同基因位点：（i）表皮素基因簇;（ii）gallidermin基因簇;（iii）编码6种酚可溶性调节蛋白（PSM）β型肽（PSM β 1-β 6）的基因簇和（iv）属于PSM β家族并编码44个残基长肽的额外基因，HTP 2388。与七种PSM β样肽（即PSM β 1-β 6和肽HTP 2388）序列相同的合成肽显示出抗菌活性。基因组序列分析还揭示了粘附素、细胞内粘附素、细胞适应性、氧化和酸胁迫耐受性等基因，这些基因可能与沙门氏菌的初始附着、定植和存活有关。capitis TE 8对人体皮肤的影响。比较基因组分析显示，S.与表皮葡萄球菌和头皮葡萄球菌相比，头皮葡萄球菌菌株表明它们在人类皮肤上更好的适应和存活中的可能作用。
Staphylococcus capitis TE8 was isolated from skin surface of a healthy human foot, and exhibited a strong antibacterial activity against Gram-positive bacteria, including Staphylococcus aureus. Whole genome sequence of S. capitis TE8 was obtained by shotgun and paired-end pyrosequencing with a coverage of 109-fold. The draft genome contains 2,516,639 bp in 8 scaffolds with 209 total contigs. The genome contains 2319 protein coding sequences, 58 tRNA and 3 rRNA. Genome sequence analysis revealed 4 distinct gene loci with the ability to encode antimicrobial peptides: (i) an epidermicin gene cluster; (ii) a gallidermin gene cluster; (iii) a gene cluster encoding six phenol soluble modulin (PSM) beta-type peptides (PSM beta 1-beta 6) and (iv) an additional gene that belonged to PSM beta family and encoded a 44 residues long peptide, HTP2388. Synthetic peptides with sequence identical to seven PSM beta-like peptides i.e. PSM beta 1-beta 6 and peptide HTP2388 showed antibacterial activity. Genome sequence also revealed genes for adhesins, intracellular adhesins, osmoadaptation, oxidative and acid stress tolerance possibly responsible for initial attachment, colonization and survival of S. capitis TE8 on human skin. Comparative genome analysis revealed presence of a gamut of genes in S. capitis strains in comparison to Staphylococcus epidermidis and Staphylococcus caprae indicating towards their possible role in better adaptation and survival on human skin.