Serum exosomal hsa_circ_0069313 has a potential to diagnose more aggressive non-small cell lung cancer
Serum exosomal hsa_circ_0069313 has a potential to diagnose more aggressive non-small cell lung cancer
复制标题
血清外泌体 hsa_circ_0069313 有潜力诊断更具侵袭性的非小细胞肺癌
DOI：
10.1016/j.clinbiochem.2022.01.005
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发表时间：
2022-02-01
影响因子：
2.8
通讯作者：
Meng, Xiaodan
中科院分区：
文献类型：
--
作者：
Chen, Yinfeng;Lou, Chengtao;Meng, Xiaodan
背景：外泌体衍生的环状RNA（circRNA）参与非小细胞肺癌（NSCLC）的癌变和发展，具有作为NSCLC诊断生物标志物的巨大潜力。方法：采用外泌体分离试剂盒分离血清外泌体，并通过蛋白质印迹、透射电镜和电位分析仪进行验证。根据circRNA阵列分析和Pubmed中发表的文献，选择了5种差异表达的外泌体circRNA，包括hsa_circ_0069313、hsa_circ_0063526、hsa_circ_0010522、hsa_circ_0048677和hsa_circ_0001946。通过qRT-PCR对上述5种circRNA的血清和血清外泌体水平进行定量。通过接受者操作特征（ROC）测试评估血清和血清外泌体hsa_circ_0069313的诊断能力。结果：血清外泌体中hsa_circ_0069313的水平显着低于匹配血清样本中的水平。相比之下，hsa_circ_0063526、hsa_circ_0010522、hsa_circ_0048677和hsa_circ_0001946的水平在健康供体的血清和血清外泌体中没有显示出统计学差异。与健康组、肺炎组和良性肺肿瘤组相比，NSCLC组中血清和血清外泌体hsa_circ_0069313的水平显着升高。此外，血清和血清外泌体 hsa_circ_0069313 可以区分良性肺肿瘤和 NSCLC，AUC 值分别为 0.803 和 0.749。有趣的是，血清外泌体hsa_circ_0069313水平较高与NSCLC的III-IV期、淋巴结转移和远处转移相关。结论：血清和血清外泌体hsa_circ_0069313具有区分NSCLC和良性肺部肿瘤的潜力。血清外泌体 hsa_circ_0069313 水平较高与 NSCLC 更具侵袭性的病理特征有关。
Background: Circular RNAs (circRNAs) derived from exosomes are involved in the carcinogenesis and development of non-small cell lung cancer (NSCLC), showing great potential to be diagnostic biomarkers for NSCLC.Methods: Serum exosomes were isolated with an exosome isolation kit, and verified by western blot, transmission electron microscopy and a potentiometric analyzer. Five differentially expressed exosomal circRNAs, including hsa_circ_0069313, hsa_circ_0063526, hsa_circ_0010522, hsa_circ_0048677 and hsa_circ_0001946, were selected based on the circRNA array analyses and the published documents in Pubmed. The serum and serum exosomal levels of the above five circRNAs were quantified by qRT-PCR. The diagnostic power of serum and serum exosomal hsa_circ_0069313 was evaluated by receiver operating characteristic (ROC) test.Results: The levels of hsa_circ_0069313 in serum exosomes were statistically lower than those in the matched serum samples. In contrast, the levels of hsa_circ_0063526, hsa_circ_0010522, hsa_circ_0048677 and hsa_circ_0001946 showed no statistical difference in the sera and serum exosomes of healthy donors. The levels of serum and serum exosomal hsa_circ_0069313 were notably elevated in the NSCLC group compared to the healthy, pneumonia and benign lung tumor groups. Furthermore, serum and serum exosomal hsa_circ_0069313 could differ benign lung tumor and NSCLC with AUC values of 0.803 and 0.749, respectively. Intriguingly, the higher levels of serum exosomal hsa_circ_0069313 were associated with stage III-IV, lymph node metastasis and distant metastasis of NSCLC.Conclusions: Serum and serum exosomal hsa_circ_0069313 have the potential to discriminate NSCLC and benign lung tumor. The higher levels of serum exosomal hsa_circ_0069313 are linked to more aggressive pathological features of NSCLC.