Multiple polyadenylation sites in a Drosophila tropomyosin gene are used to generate functional mRNAs.
Multiple polyadenylation sites in a Drosophila tropomyosin gene are used to generate functional mRNAs.
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果蝇原肌球蛋白基因中的多个聚腺苷酸化位点用于生成功能性 mRNA。
DOI：
10.1093/nar/13.5.1763
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发表时间：
1985
影响因子：
14.9
通讯作者：
Storti,RV
中科院分区：
文献类型：
--
作者：
Boardman,M;Basi,GS;Storti,RV
果蝇的原肌球蛋白1基因在胚胎肌和胸肌中选择性剪接，产生两种大小的rna。通过Northern blot分析，胚胎RNA类显示出1.3 kb的宽RNA带和1.6 kb的更清晰，较少丰富的RNA带。另一方面，胸椎类rna由1.7 kb的宽杂交带和1.9 kb的更清晰的杂交带组成。每个大小类别的RNA编码不同的原肌球蛋白亚型。这两类可选择剪接的rna利用相同的基因3 '末端外显子。该外显子的DNA序列揭示了一个由多个聚腺苷化信号（AAUAAA）或聚腺苷化样信号组成的簇。我们通过S1核酸酶保护分析表明，至少有5个，可能有7个这些聚腺苷酸或聚腺苷酸样序列与活胚和胸椎mRNA切割加工位点有关。这些S1位点中有6个聚集在119bp以内，第7个位于下游255bp。聚腺苷化样信号序列中至少有一个是不寻常的AACAAA序列。此外，我们还发现这些mrna在体外可以合成肌原肌球蛋白。
The gene encoding muscle tropomyosin I inDrosophilais alternatively spliced in embryonic and thoracic muscle to generate two size classes of RNAs. By Northern blot analysis, the embryonic RNA class shows a broad RNA band of hybridization of 1.3 kb and a more sharply defined, less abundant RNA band at 1.6 kb. The thoracic class of RNAs, on the other hand, consists of a broad hybridization band at 1.7 kb and a more sharply defined band at 1.9 kb. Each size class of RNA encodes a different tropomyosin isoform. The two classes of alternatively spliced RNAs utilize the same 3′ terminal exon of the gene. The DNA sequence of this exon reveals a cluster of several polyadenylatlon signals (AAUAAA) or polyadenylation-like signals. We show here by S1 nuclease protection analysis that at least five and possibly seven of these polyadenylatlon or polyadenylation-like sequences are associated within vivoembryonic and thoracic mRNA cleavage processing sites. Six of these S1 sites are clustered within 119 bp and a seventh is located 255 bp downstream. At least one of the polyadenylation-like signal sequences appears to be an unusual AACAAA sequence. In addition we also show that these mRNAs functionin vitroto synthesize muscle tropomyosins.