FATTY-ACID OXIDATION IN BONE TISSUE AND BONE-CELLS IN CULTURE - CHARACTERIZATION AND HORMONAL INFLUENCES
FATTY-ACID OXIDATION IN BONE TISSUE AND BONE-CELLS IN CULTURE - CHARACTERIZATION AND HORMONAL INFLUENCES
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DOI：
10.1042/bj2480129
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发表时间：
1987-11-15
影响因子：
4.1
通讯作者：
FLEISCH, H
中科院分区：
文献类型：
--
作者：
ADAMEK, G;FELIX, R;FLEISCH, H
在培养的大鼠颅骨和培养的细胞群中研究了脂肪酸氧化及其激素调节。后者是从新生大鼠的颅骨中通过胶原酶连续时间依赖性消化获得的，产生八个细胞群：早期的细胞群主要含有成纤维细胞，中间的细胞群为成骨细胞样，晚期的细胞群为成骨细胞-骨细胞样。在颅盖中，通过向含有 10% (v/v) 胎牛血清的培养基中添加 0.1 mM 和 1.0 mM 棕榈酸酯来增加脂肪酸氧化。体外添加甲状旁腺素后或体内注射时均未发现任何影响。发现通过连续消化获得的所有细胞群都氧化棕榈酸酯，其中成骨细胞样细胞显示出比其他细胞群更低的氧化率。甲状旁腺素和降钙素对脂肪酸氧化没有影响。 1-100 nM 的 1,25-二羟基胆钙化醇和 100 nM 的 24,25-二羟基胆钙化醇主要在富含成骨细胞样细胞的群体中增加氧化。 1.6μM的胰岛素减少了富含成骨细胞样细胞的细胞群和晚期骨细胞部分中的它。然而，胰高血糖素没有效果。该系统中脂肪酸氧化提供的能量约为。 40-80%的葡萄糖代谢，表明该事件可能在骨的能量代谢中具有重要意义。
Fatty acid oxidation and its hormonal modulation were investigated in cultured rat calvaria and in cultivated cell populations. The latter were obtained from calvaria of newborn rats by sequential time-dependent digestion with collagenase, yielding eight cell populations: the early ones containing mainly fibroblasts, the middle ones being osteoblast-like, and late ones osteoblast-osteocyte-like. In calvaria, fatty acid oxidation was increased by adding 0.1 mM- and 1.0 mM-palmitate to the medium, containing 10% (v/v) fetal-calf serum. No effect was found after parathyrin addition in vitro or when injected in vivo. All cell populations obtained by sequential digestion were found to oxidize palmitate, whereby the osteoblast-like cells showed a lower oxidation rate than the other populations. Both parathyrin and calcitonin had no effect on fatty acid oxidation. 1,25-Dihydroxycholecalciferol at 1-100 nM and 24,25-dihydroxycholecalciferol at 100 nM increased oxidation primarily in the population enriched with osteoblast-like cells. Insulin at 1.6 .mu.M diminished it in the cell populaitons enriched with osteoblast-like cells and in the late bone-cell fraction. However, glucagon had no effect. The energy provided by fatty acid oxidation in this system is approx. 40-80% of glucose metabolism, suggesting that this event may be of importance in the energy metabolism of bone.