Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary
Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary
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DOI：
10.1242/dev.01093
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发表时间：
2004-05-01
期刊：
影响因子：
4.6
通讯作者：
Bally-Cuif, L
中科院分区：
文献类型：
--
作者：
Geling, A;Plessy, C;Bally-Cuif, L
脊椎动物和无脊椎动物的神经发生在时间和空间上受到严格的控制，涉及正调控和负调控。我们在这里报道bHLH因子Her5作为一种前模式基因，在斑马鱼神经板的中脑/后脑边界处阻止神经发生。这涉及在一个独立于Notch信号传导的过程中选择性抑制神经原蛋白1 （ngn1）和coe2 mRNA表达，并且抑制ngn1或coe2表达足以阻止神经元在中脑-后脑边界的分化。一个ngn1转基因忠实地响应Her5，对该转基因的缺失分析在ngn1上游增强子中发现了一个E-box，该E-box是Her5抑制所必需的。总之，我们的数据表明Her5在斑马鱼神经板中作为前模式因子的空间定义中的作用，其方式类似于其果蝇同源毛。
Neurogenesis in both vertebrates and invertebrates is tightly controlled in time and space involving both positive and negative regulators. We report here that the bHLH factor Her5 acts as a prepattern gene to prevent neurogenesis in the anlage of the midbrain/hindbrain boundary in the zebrafish neural plate. This involves selective suppression of both neurogenin1 (ngn1) and coe2 mRNA expression in a process that is independent of Notch signalling, and where inhibition of either ngn1 or coe2 expression is sufficient to prevent neuronal differentiation across the midbrain-hindbrain boundary. A ngn1 transgene faithfully responds to Her5 and deletion analysis of the transgene identifies an E-box in a ngn1 upstream enhancer to be required for repression by Her5. Together our data demonstrate a role of Her5 as a prepattern factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy.