A MOLECULAR-BASED APPROACH FOR EXAMINING RESPONSES OF EUKARYOTES IN MICROCOSMS TO CONTAMINANT-SPIKED ESTUARINE SEDIMENTS
A MOLECULAR-BASED APPROACH FOR EXAMINING RESPONSES OF EUKARYOTES IN MICROCOSMS TO CONTAMINANT-SPIKED ESTUARINE SEDIMENTS
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DOI：
10.1002/etc.2450
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发表时间：
2014-02-01
影响因子：
4.1
通讯作者：
Matthews, Robin A.
中科院分区：
文献类型：
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作者：
Chariton, Anthony A.;Ho, Kay T.;Matthews, Robin A.
大多数污染物的生态毒理学信息仅限于少数分类群，这些分类群通常限于易于从试验介质中提取并易于识别的相对哈代生物。DNA测序的进展现在可以提供底栖无脊椎动物多样性的全面视图。作者应用454焦磷酸测序技术，研究了暴露于三氯生浓度升高的沉积物的微宇宙中底栖生物群落的反应，终点是已成功垂直迁移通过受操纵沉积物的真石脂群落。与3种处理（对照三氯生、低三氯生[14 mg/kg]和高三氯生[180 mg/kg]）相关的生物群落分为3组：对照/低（n=6个对照和4个低）、中度（n=2个低）和高（n=5个高）。一份样本作为离群值丢弃。作为对三氯生的反应，最显著的变化是后生动物操作分类单位（OTU）数量的损失，这表明对照/低和中等组，这在与色壳纲和双壳纲以及动喙门相关的分类群范围内最为明显。作者还描述了一系列其他分类群，帮助群体之间的歧视;比较结果与传统上获得的小型和大型动物群落从同一实验中获得;并说明了一些与分子和传统方法相关的优点和局限性。所描述的方法说明了扩增子测序的能力，提供生态相关的信息，可用于加强沉积群落如何应对一系列环境压力的理解。(c)2014年SETAC
Ecotoxicological information for most contaminants is limited to a small number of taxa, and these are generally restricted to comparatively hardy organisms that are readily extractable from test media and easily identifiable. Advances in DNA sequencing can now provide a comprehensive view of benthic invertebrate diversity. The authors applied 454 pyrosequencing to examine the responses of benthic communities in microcosms exposed to sediments with elevated concentrations of triclosan, the endpoint being eukaryl communities that have successfully vertically migrated through the manipulated sediments. The biological communities associated with the 3 treatments (control triclosan, low triclosan [14mg/kg], and high triclosan [180mg/kg]) clustered into 3 groups: control/low (n=6 controls and 4 low), moderate (n=2 low), and high (n=5 high). One sample was discarded as an outlier. The most pronounced change as a response to triclosan was the loss of number of metazoan operational taxonomic units (OTUs), indicative of the control/low and moderate groups, with this being most evident in the range of taxa associated with the classes Chromadorea and Bivalvia and the phylum Kinorhyncha. The authors also describe a range of other taxa that aided discrimination between the groups; compare findings with traditionally obtained meio- and macrofaunal communities obtained from the same experiment; and illustrate some of the advantages and limitations associated with both the molecular and traditional approaches. The described approach illustrates the capacity for amplicon sequencing to provide ecologically relevant information that can be used to strengthen an understanding of how sedimentary communities respond to a range of environmental stressors. (c) 2014 SETAC