Investigating the Role of Glutathione S- Transferase Genes, Histopathological and Molecular Subtypes, Gene-Gene Interaction and Its Susceptibility to Breast Carcinoma in Ethnic North- Indian Population.
Investigating the Role of Glutathione S- Transferase Genes, Histopathological and Molecular Subtypes, Gene-Gene Interaction and Its Susceptibility to Breast Carcinoma in Ethnic North- Indian Population.
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研究谷胱甘肽S-转移酶基因，组织病理学和分子亚型，基因基因相互作用及其对北印度种群中乳腺癌的敏感性的作用。
DOI：
10.31557/apjcp.2022.23.10.3481
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发表时间：
2022-10-01
期刊：
影响因子：
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通讯作者：
Kumar, Munish
中科院分区：
文献类型：
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作者：
Gautam, Priyanka;Feroz, Zainab;Tiwari, Sonia;Vijayraghavalu, Sivakumar;Shukla, Girish C;Kumar, Munish
乳腺癌是一种遗传性和临床异质性疾病，包括基因-基因和基因-环境成分之间复杂的相互作用。本研究旨在探讨谷胱甘肽S转移酶(GSTS)基因多态性是否与BC易感性有关。我们根据分子分类和显微组织学分析进一步评估了四种BC亚型的出现频率，以研究浸润性导管癌(IDC)的分级。采用多重聚合酶链式反应和聚合酶链式反应-限制性片段长度多态性方法检测北印度BC患者GST基因的多态性。105例慢性胆管炎患者和145例健康对照参加本研究。采用Logistic回归分析，计算优势比(OR)和95%可信区间(CI)。我们的研究结果显示，GSTM1零基因(OR=2.231；95%CI=1.332-3.737；p值=0.002)与北印度裔人群的BC风险显著相关。然而，北印度人患BC易感性的风险似乎与GSTT1零基因无关。GSTP1Val/Val基因型(OR=1.545；CI=0.663~3.605；P值=0.314)也是BC的易感基因。三种高危GST基因关联的基因-基因交互作用的组合进一步证实了该地区患BC的风险增加。本研究结果提示，GSTP1基因GSTM1和rs1695的多态性可能影响北印度女性BC的发育。因此，应将GSTM1和GSTP1基因的筛查作为预防措施，用于BC的早期研究。
Breast Cancer (BC) is a genetically and clinically heterogeneous disease including complex interactions between gene-gene and gene-environment components. This study aimed, to explore whether the Glutathione S- transferase (GSTs) gene polymorphism has role in BC susceptibility. We further evaluated the frequency of four subtypes of BC based on molecular classification followed by microscopic histological analysis to study the grades of invasive ductal carcinoma (IDC). Polymorphism in GST genes in North-Indian BC patients was assessed by multiplex-PCR and PCR-RFLP methods. 105 BC patients and 145 healthy controls were enrolled for this study. Data was analyzed by calculating the odds ratio (OR) and 95% CI from logistic regression analyses. Our findings revealed that GSTM1 null genotype (OR = 2.231; 95% CI = 1.332–3.737; p-value= 0.002) is significantly associated to BC risk in ethnic North- Indian population. However, the risk for BC susceptibility in North–Indians does not appear to be associated with GSTT1 null genotype. The GSTP1 (Val/Val) genotype (OR=1.545; CI=0.663-3.605; p-value= 0.314) was also found to be susceptible for BC risk. Combination of three high risk GST genotypes association exhibiting gene-gene interaction further confirmed the increased risk to BC in this region. The results of present study indicated that polymorphism in GSTM1 and rs1695 of GSTP1 genes may influence BC development among North-Indian women. Thus, the screening of GSTM1 and GSTP1 gene should be recommended for the earlier investigation for BC as a precautionary measure.