Kinetic Studies of Lipid Oxidation Induced by Hemoglobin Measured by Consumption of Dissolved Oxygen in a Liposome Model System
Kinetic Studies of Lipid Oxidation Induced by Hemoglobin Measured by Consumption of Dissolved Oxygen in a Liposome Model System
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DOI：
10.1021/jf9013394
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发表时间：
2009-09-09
影响因子：
6.1
通讯作者：
Storro, Ivar
中科院分区：
文献类型：
--
作者：
Carvajal, Ana Karina;Rustad, Turid;Storro, Ivar
研究了血红蛋白（Hb）和脂质浓度、pH、温度和不同抗氧化剂对海洋磷脂脂质体血红素介导的脂质氧化的影响。通过溶解氧消耗测定脂质氧化速率。采用Michaelis-Menten动力学模拟血红素在不同血红蛋白和脂质浓度下介导的脂质氧化。以cod和牛Hb为促氧化剂的最大反应速率（V-max）分别为66.2 +/- 3.4和56.6 +/- 3.4 μ M/min。与cod和牛Hb反应的Michaelis-Menten常数（K-m）分别为0.67 +/- 0.09和1.2 +/- 0.2 μ M。氧摄取速率与脂质浓度关系的V-max为43.2 +/- 1.5 μ M/min， K-m为0.93 +/- 0.14 mg/mL。温度的影响遵循阿伦尼乌斯动力学，cod和牛Hb的活化能无显著差异。牛Hb诱导的脂质氧化率在pH为6时最高。乙二胺四乙酸（EDTA）对血红素介导的脂质氧化无显著影响，但α -生育酚和虾青素具有良好的抗氧化作用。作为促氧化剂，铁和血红蛋白之间存在动力学差异，抗氧化剂的效果取决于系统中的促氧化剂。
The effect of hemoglobin (Hb) and lipid concentration, pH, temperature, and different antioxidants on heme-mediated lipid oxidation of liposomes from marine phospholipids was studied. The rate of lipid oxidation was measured by consumption of dissolved oxygen. Heme-mediated lipid oxidation at different Hb and lipid concentrations was modeled by Michaelis-Menten kinetics. The maximum rate (V-max) for the reaction with cod and bovine Hb as a pro-oxidant was 66.2 +/- 3.4 and 56.6 +/- 3.4 mu M/min, respectively. The Michaelis-Menten constant (K-m) for the reaction with cod and bovine Hb was 0.67 +/- 0.09 and 1.2 +/- 0.2 mu M, respectively. V-max for the relationship between the oxygen uptake rate and lipid concentration was 43.2 +/- 1.5 mu M/min, while the K-m was 0.93 +/- 0.14 mg/mL. The effect of the temperature followed Arrhenius kinetics, and there was no significant difference in activation energy between cod and bovine Hb. The rate of lipid oxidation induced by bovine Hb was highest around pH 6. Ethylenediaminetetraacetic acid (EDTA) had no significant effect on heme-mediated lipid oxidation, but alpha-tocopherol and astaxanthin worked well as antioxidants. Kinetic differences were found between iron and Hb as pro-oxidants, and the efficacy of the antioxidants depended upon the pro-oxidant in the system.