Isolation of a Novel Orientia Species (O. chuto sp nov.) from a Patient Infected in Dubai
Isolation of a Novel Orientia Species (O. chuto sp nov.) from a Patient Infected in Dubai
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DOI：
10.1128/jcm.01526-10
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发表时间：
2010-12-01
影响因子：
9.4
通讯作者：
Stenos, John
中科院分区：
文献类型：
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作者：
Izzard, Leonard;Fuller, Andrew;Stenos, John
2006年7月，一名从阿拉伯联合酋长国（UAE）迪拜返回的澳大利亚游客患上了急性丛林斑疹伤寒。她的体征和症状包括发烧、肌痛、头痛、皮疹和焦痂。恙虫病东方体血清学显示，配对血清集合中的抗体滴度增加了 4 倍（1: 512 至 1: 8,192），其中血清对 Gilliam 株抗原的反应最强。通过对抗生素治疗前采集的患者急性血液进行体外培养，分离出东方菌属物种。对16S rRNA基因（rrs）、部分56-kDa基因和完整开放阅读框47-kDa基因进行了基因测序，并对这个新的Orientia sp.进行了比较。分离到先前表征的菌株表现出显着的序列多样性。与新 Orientia sp 的 rrs 序列最接近的同源性。该分离株与恙虫病 3 株（池田、加藤和卡普），核苷酸序列相似性达 98.5%。与47 kDa基因序列同源性最接近的是恙虫病菌株Gilliam，核苷酸相似性为82.3%，而与56 kDa基因序列同源性最接近的是恙虫病菌株TA686，核苷酸相似性为53.1%。分子差异和地理上独特的起源使我们相信这种生物体应该被视为一个新物种。因此，我们提出了“Orientia chuto”这个名字，这个物种的原型菌株是Dubai菌株，以患者感染的地点命名。
In July 2006, an Australian tourist returning from Dubai, in the United Arab Emirates (UAE), developed acute scrub typhus. Her signs and symptoms included fever, myalgia, headache, rash, and eschar. Orientia tsutsugamushi serology demonstrated a 4-fold rise in antibody titers in paired serum collections (1: 512 to 1: 8,192), with the sera reacting strongest against the Gilliam strain antigen. An Orientia species was isolated by the in vitro culture of the patient's acute blood taken prior to antibiotic treatment. The gene sequencing of the 16S rRNA gene (rrs), partial 56-kDa gene, and the full open reading frame 47-kDa gene was performed, and comparisons of this new Orientia sp. isolate to previously characterized strains demonstrated significant sequence diversity. The closest homology to the rrs sequence of the new Orientia sp. isolate was with three strains of O. tsutsugamushi (Ikeda, Kato, and Karp), with a nucleotide sequence similarity of 98.5%. The closest homology to the 47-kDa gene sequence was with O. tsutsugamushi strain Gilliam, with a nucleotide similarity of 82.3%, while the closest homology to the 56-kDa gene sequence was with O. tsutsugamushi strain TA686, with a nucleotide similarity of 53.1%. The molecular divergence and geographically unique origin lead us to believe that this organism should be considered a novel species. Therefore, we have proposed the name "Orientia chuto," and the prototype strain of this species is strain Dubai, named after the location in which the patient was infected.