Experimental verification of a theory for the time-resolved fluorescence spectroscopy of thick tissues
Experimental verification of a theory for the time-resolved fluorescence spectroscopy of thick tissues
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DOI：
10.1364/ao.36.000116
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发表时间：
1997-01-01
期刊：
影响因子：
1.9
通讯作者：
Gratton, E
中科院分区：
文献类型：
--
作者：
Cerussi, AE;Maier, JS;Gratton, E
荧光光谱为近红外组织成像和生理相关光谱法提供了潜在的对比度增强。我们提出荧光光子迁移模型，并通过频域测量测量其定量预测能力，该测量涉及均匀的多散射组织幻像（具有含有荧光酚（Rhodamine b）的近红外组织的光学特性（具有类似于近红外组织的光学特性） 。在证明了模型的有效性之后，我们探索了其从多散射培养基中恢复荧光团光谱特性的能力。在没有散射的情况下，将绝对量子产率和荧光团的寿命在独立测量的值的几％之内。在不使用参考荧光团的情况下，完成了两种测量。此外，该模型可以准确预测散射介质中的荧光发射光谱。讨论了这些绝对测量的寿命，量子产率，浓度和发射光谱的含义。 （c）1997美国光学学会
Fluorescence spectroscopy provides potential contrast enhancement for near-infrared tissue imaging and physiologically correlated spectroscopy. We present a fluorescence photon migration model and test its quantitative predictive capabilities with a frequency-domain measurement that involves a homogeneous multiple-scattering tissue phantom (with optical properties similar to those of tissue in the near infrared) that contains a fluorophore (rhodamine B). After demonstrating the validity of the model, we explore its ability to recover the fluorophore's spectral properties from within the multiple-scattering medium. The absolute quantum yield and the lifetime of the fluorophore are measured to within a few percent of the values measured independently in the absence of scattering. Both measurements are accomplished without the use of reference fluorophores. In addition, the model accurately predicts the fluorescence emission spectrum in the scattering medium. Implications of these absolute measurements of lifetime, quantum yield, concentration, and emission spectrum from within multiple-scattering media are discussed. (C) 1997 Optical Society of America