Insights into Australian Bat Lyssavirus in Insectivorous Bats of Western Australia
Insights into Australian Bat Lyssavirus in Insectivorous Bats of Western Australia
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DOI：
10.3390/tropicalmed4010046
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发表时间：
2019-03-01
影响因子：
2.9
通讯作者：
O'Dea, Mark
中科院分区：
文献类型：
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作者：
Prada, Diana;Boyd, Victoria;O'Dea, Mark
澳大利亚蝙蝠溶血病毒(ABLV)是一种已知的蝙蝠、人和马神经系统疾病的病原体。它是从四种翼足类蝙蝠和一种微蝙蝠(Saccolaimus Flaviventris)中分离出来的。到目前为止，ABLV监测主要是被动的，主动监测集中在澳大利亚东部和北部的蝙蝠种群。因此，该国大片地区的地区性ABLV信息很少。为了更好地告知与人与蝙蝠相互作用相关的当地公共卫生风险，本研究描述了西澳大利亚西南植物省微型蝙蝠群落中溶状病毒的流行情况。我们使用有针对性的实时PCR方法检测了代表12种微蝙蝠的839份口腔拭子中的病毒RNA脱落，这些样本是在2016-2018年连续两个夏天采集的。此外，我们还通过Luminex(R)试验测试了649个血清样本对溶血病毒抗原的反应性。在任何样本中均未检测到活动性溶血病毒感染。在6个物种的19个个体中检测到溶状病毒抗体，两年来粗略流行率为2.9%(95%可信区间：1.8-4.5%)。此外，我们首次记录了在两个Nyctophurus物种和Falsistrellus mackenziei中暴露的溶酶病毒。
Australian bat lyssavirus (ABLV) is a known causative agent of neurological disease in bats, humans and horses. It has been isolated from four species of pteropid bats and a single microbat species (Saccolaimus flaviventris). To date, ABLV surveillance has primarily been passive, with active surveillance concentrating on eastern and northern Australian bat populations. As a result, there is scant regional ABLV information for large areas of the country. To better inform the local public health risks associated with human-bat interactions, this study describes the lyssavirus prevalence in microbat communities in the South West Botanical Province of Western Australia. We used targeted real-time PCR assays to detect viral RNA shedding in 839 oral swabs representing 12 species of microbats, which were sampled over two consecutive summers spanning 2016-2018. Additionally, we tested 649 serum samples via Luminex (R) assay for reactivity to lyssavirus antigens. Active lyssavirus infection was not detected in any of the samples. Lyssavirus antibodies were detected in 19 individuals across six species, with a crude prevalence of 2.9% (95% CI: 1.8-4.5%) over the two years. In addition, we present the first records of lyssavirus exposure in two Nyctophilus species, and Falsistrellus mackenziei.