miRNA-214 Inhibits Cellular Proliferation and Migration in Glioma Cells Targeting Caspase 1 Involved in Pyroptosis.
miRNA-214 Inhibits Cellular Proliferation and Migration in Glioma Cells Targeting Caspase 1 Involved in Pyroptosis.
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miRNA-214 靶向参与焦亡的 Caspase 1 抑制神经胶质瘤细胞的细胞增殖和迁移
DOI：
10.3727/096504016x14813859905646
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发表时间：
2017-07-05
影响因子：
3.1
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Jiang Z;Yao L;Ma H;Xu P;Li Z;Guo M;Chen J;Bao H;Qiao S;Zhao Y;Shen J;Zhu M;Meyers C;Ma G;Xie C;Liu L;Wang H;Zhang W;Dong Q;Shen H;Lin Z
焦亡是一种由半胱天冬酶1活性介导的促炎性程序性细胞死亡，发生在几种类型的真核肿瘤细胞中，包括神经胶质瘤。microRNAs（miRNAs）是一种内源性非编码小分子RNA，在胶质瘤治疗中具有重要作用。然而，miRNAs是否调节胶质瘤中的焦亡的问题仍然未知。目前的研究发现，在胶质瘤组织和胶质瘤细胞系U87和T98 G中，caspase 1的表达显著增加，而miR-214的表达显著下调。荧光素酶报告基因分析将caspase 1识别为miR-214的靶基因。这些结果表明，miR-214可以通过调节caspase 1介导的胶质瘤U87和T98 G细胞的焦亡来抑制细胞增殖和迁移，并可能为干预胶质瘤提供新的治疗方法。
Pyroptosis is a type of proinflammatory programmed cell death mediated by caspase 1 activity and occurs in several types of eukaryotic tumor cells, including gliomas. MicroRNAs (miRNAs), small endogenous noncoding RNAs, have been demonstrated to be advantageous in glioma therapy. However, the question of whether miRNAs regulate pyroptosis in glioma remains unknown. The current study found that caspase 1 expression was substantially increased in both glioma tissues and glioma cell lines, U87 and T98G, while miR-214 expression was significantly downregulated. Luciferase reporter assay recognized caspase 1 as a target gene of miR-214. These findings demonstrate that miR-214 could inhibit cell proliferation and migration through the regulation of pyroptosis intermediated by caspase 1 in glioma U87 and T98G cells and may suggest a novel therapeutic for the intervention of glioma.