MXene-derived Ti3C2 quantum dots-based ratiometric fluorescence probe for ascorbic acid and acid phosphatase determination
MXene-derived Ti3C2 quantum dots-based ratiometric fluorescence probe for ascorbic acid and acid phosphatase determination
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DOI：
10.1016/j.microc.2023.108397
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发表时间：
2023-01-09
影响因子：
4.8
通讯作者：
Cen，Yao
中科院分区：
文献类型：
--
作者：
Ren，Dandan;Cheng，Xia;Cen，Yao
作为一种新兴的零维纳米材料，由二维过渡金属碳化物衍生的MXene量子点近年来引起了人们的极大兴趣。将二维MXene转变为几纳米的零维结构使这些最近发现的材料具有特殊的量子尺寸效应和显着的光致发光特性。因此，我们通过小分子胺辅助溶剂热策略合成了具有明亮蓝色荧光的N掺杂Ti 3C 2 MXene量子点（N-Ti 3C 2 MQDs）。基于N-Ti 3C 2 MQDs和邻苯二胺（OPD）的优良光学性能，我们构建了一种新型的比率荧光生物传感器，用于抗坏血酸（AA）和酸性磷酸酶（ACP）的测定。该比例策略是基于N-Ti 3C 2 MQDs与OPD氧化物之间的内过滤效应而建立的。AA和ACP在2-240 μM和0.15- 3.75U/L范围内灵敏地进行了荧光分析，检测限分别低至0.82 μM和0.02U/L。此外，该荧光传感器测定AA和ACP的响应时间小于1.5小时。AA和ACP测定的日内和日间相对标准偏差均小于8.3%，表明该传感器具有良好的重现性。此外，在紫外光照射下，随着待测物浓度的增加，其颜色发生了丰富的变化，显示出很大的视觉检测和现场应用的潜力。该方法被进一步用于筛选潜在的ACP抑制剂，表明该探针在临床应用中具有很大的潜力。
As a burgeoning zero-dimensional nanomaterial, MXene quantum dots which are derived from two-dimensional transition-metal carbides have attracted a lot of interest lately. The transformation of two-dimensional MXene into zero-dimensional structures of a few nanometers enables these recently discovered materials to possess an exceptional quantum size effect and remarkable photoluminescence characteristics. Hence, we synthesized N doped Ti3C2MXene quantum dots (N-Ti3C2MQDs) exhibiting bright blue fluorescence through small-molecule amine-assisted solvothermal strategy. In this work, we constructed a novel ratiometric fluorescence biosensor for the determination of ascorbic acid (AA) and acid phosphatase (ACP) based on the excellent optical performance of N-Ti3C2MQDs and combinedo-phenylenediamine (OPD). This ratiometric strategy was established based on the inner filter effect between N-Ti3C2MQDs and the oxide of OPD. Sensitive fluorometric analyses of AA and ACP were achieved successfully in the respective ranges of 2–240 μM and 0.15–3.75 U/L with respective limits of detection down to 0.82 μM and 0.02 U/L. Moreover, the response time of this fluorescence sensor for AA and ACP determination was less than 1.5 h. The intraday and interday relative standard deviations for AA and ACP assay were all below 8.3 %, suggesting the good reproducibility of this sensor. In addition, the profuse color changed under UV light with analytes concentrations increased and exhibited great potential for visual detection and on-site application. The proposed method was further utilized to screen of potential ACP inhibitors, indicating that this probe has great potential in clinical practice.