Vitamin A distribution and content in tissues of the lamprey, Lampetra japonica.
Vitamin A distribution and content in tissues of the lamprey, Lampetra japonica.
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DOI：
10.1002/ar.a.10345
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发表时间：
2004-02
期刊：
影响因子：
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通讯作者：
H. L. Wold;K. Wake;N. Higashi;Da-Ren Wang;N. Kojima;K. Imai;R. Blomhoff;H. Senoo
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文献类型：
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作者：
H. L. Wold;K. Wake;N. Higashi;Da-Ren Wang;N. Kojima;K. Imai;R. Blomhoff;H. Senoo
用氯化金染色、荧光显微镜检测特异性维生素A自体荧光、电子显微镜和高效液相色谱(HPLC)等形态学方法，分析了维生素A(视黄醇和视黄酯)在七鳃鳗组织中的分布和含量。肝星状细胞显示大量维生素A储存在细胞质中的脂滴中。雌鳗和雄鳗的肠、肾、鳃和心脏中都存在类似的储存维生素A的细胞。氯化金染色法、荧光显微镜、透射电子显微镜和高效液相色谱法测定视黄醇的形态数据一致。用高效液相色谱法测定的总视黄醇含量在肠道中最高。肝脏和肾脏中的维生素A含量分别位居第二和第三。这些储存维生素A的细胞不是上皮细胞，而是中胚层来源的细胞。我们提出的假设是，这些细胞属于星状细胞系统(家族)，该系统储存维生素A，并调节七鳃鳗体内维生素的动态平衡。皮肤和躯体肌肉中的成纤维细胞储存的维生素A很少。这些结果表明，七鳃鳗内脏和中间中胚层来源的细胞(星状细胞)和体细胞和背部中胚层来源的细胞(成纤维细胞)储存维生素A的能力存在差异。来自内脏和中间中胚层的星状细胞具有较高的储存能力，而来自体细胞和背部中胚层的成纤维细胞储存维生素A的能力较低。
Vitamin A (retinol and retinyl ester) distribution and content in tissues of a lamprey (Lampetra japonica) were analyzed by morphological methods, namely, gold chloride staining, fluorescence microscopy to detect specific vitamin A autofluorescence, and electron microscopy, as well as high-performance liquid chromatography (HPLC). Hepatic stellate cells showed an abundance of vitamin A stored in lipid droplets in their cytoplasm. Similar cells storing vitamin A were present in the intestine, kidney, gill, and heart in both female and male lampreys. Morphological data obtained by gold chloride staining method, fluorescence microscopy, transmission electron microscopy, and HPLC quantification of retinol were consistent. The highest level of total retinol measured by HPLC was found in the intestine. The second and third highest concentrations of vitamin A were found in the liver and the kidney, respectively. These vitamin A-storing cells were not epithelial cells, but mesoderm-derived cells. We propose as a hypothesis that these cells belong to the stellate cell system (family) that stores vitamin A and regulates homeostasis of the vitamin in the whole body in the lamprey. Fibroblastic cells in the skin and somatic muscle stored little vitamin A. These results indicate that there is difference in the vitamin A-storing capacity between the splanchnic and intermediate mesoderm-derived cells (stellate cells) and somatic and dorsal mesoderm-derived cells (fibroblasts) in the lamprey. Stellate cells derived from the splanchnic and intermediate mesoderm have high capacity and fibroblasts derived from the somatic and dorsal mesoderm have low capacity for the storage of vitamin A in the lamprey.