Open-source, 3D-printed Peristaltic Pumps for Small Volume Point-of-Care Liquid Handling
Open-source, 3D-printed Peristaltic Pumps for Small Volume Point-of-Care Liquid Handling
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DOI：
10.1038/s41598-020-58246-6
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发表时间：
2020-01-31
影响因子：
4.6
通讯作者：
Steward, Robert, Jr.
中科院分区：
文献类型：
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作者：
Behrens, Michael R.;Fuller, Haley C.;Steward, Robert, Jr.
微流控技术经常被用作现场诊断工具，以缩短诊断时间并改善临床环境中的患者治疗结果。这些微流体设备通常设计为与用于液体处理的外围设备一起操作，这增加了这些系统的成本和复杂性，并降低了它们在低资源医疗保健应用中广泛采用的潜力。在这里，我们提出了一种低成本（约 120 美元）开源蠕动泵，由三维 (3D) 打印零件和通用硬件组合而成，适合与微流体设备一起部署以进行即时诊断。该泵可接受尺寸范围为 1.5 至 3 mm 的常用硅橡胶管，并且能够产生高达 1.6 mL min(-1) 的流量。该设备采用 Arduino 微控制器进行编程，允许自定义流量曲线，以适应各种低容量液体处理应用，包括精密液体等分、微流体内的流量控制以及生成生理相关力以研究微流体系统内的细胞力学生物学。
Microfluidic technologies are frequently employed as point-of-care diagnostic tools for improving time-to-diagnosis and improving patient outcomes in clinical settings. These microfluidic devices often are designed to operate with peripheral equipment for liquid handling that increases the cost and complexity of these systems and reduces their potential for widespread adoption in low resource healthcare applications. Here, we present a low-cost (similar to$120), open-source peristaltic pump constructed with a combination of three dimensional (3D)-printed parts and common hardware, which is amenable to deployment with microfluidic devices for point-of-care diagnostics. This pump accepts commonly available silicone rubber tubing in a range of sizes from 1.5 to 3 mm, and is capable of producing flow rates up to 1.6 mL min(-1). This device is programmed with an Arduino microcontroller, allowing for custom flow profiles to fit a wide range of low volume liquid handling applications including precision liquid aliquoting, flow control within microfluidics, and generation of physiologically relevant forces for studying cellular mechanobiology within microfluidic systems.